巨核细胞酶标染色法在贫血鉴别诊断中的应用价值  

The significance of micromegakaryocytes enzyme linked immunoa-bsorbent assay in differential diagnosis of anemia

作　　者：杨颉[1] 孟冬梅[1] 宋文斌[1] 孙晓玲 陈兵[1] 

机构地区：[1]青岛大学医学院附属医院血液科,山东青岛266003 [2]青岛平度市中医院检验科

出　　处：《临床血液学杂志》2007年第2期96-98,共3页Journal of Clinical Hematology

摘　　要：目的:探讨酶标法检测骨髓小巨核细胞在贫血诊断和鉴别诊断中的意义。方法:单纯性贫血患者共计123例,其中,骨髓增生异常综合征26例,缺铁性贫血20例,巨幼细胞性贫血10例,溶血性贫血18例,多发性骨髓瘤21例,骨髓纤维化9例,肾性贫血7例,纯红细胞再生障碍性贫血3例,慢性病贫血5例,慢性再生障碍性贫血4例。分别应用巨核细胞酶标染色法(APAAP)和瑞特染色法,计数患者骨髓涂片中巨核细胞的总数(包括小巨核细胞),并对小巨核细胞进行分类计数。结果:APAAP法较瑞特染色法检测的巨核细胞的数量多,差异有统计学意义(P<0．05);且APAAP法较瑞特染色法检测小巨核细胞的阳性率高,差异有统计学意义(P< 0．05)。结论:APAAP法检测骨髓小巨核细胞,其结果明显优于瑞特染色法,阳性率高且特异性强,在单纯性贫血的鉴别诊断中有重要的应用价值。Objective：To investigate the significance of micromegakaryocytes enzyme linked immunoabsorbent assay in differential diagnosis of anaemia. Method： Bone marrow smears of 123 patients with anemia, including 26 cases myelodysplastic syndrome, 20 cases iron deficiency anemia, 10 cases megaloblastic anemia,18 cases hemolyt- ic anemia, 21 cases multiple myeloma, 9 cases myelofibrosis, 7 cases renal anemia, 3 cases pure red cell aplastic anemia, 5 cases anemia of chronic disease,4 cases chronic aplastic anemia, were assayed by APAAP technique and Wright＇s stain, respectively. The amount of rnegakaryocytes and the amount and type of micromegakaryocytes were analyzed. Result：The averge amount and the positive rate of megakaryocytes assayed by APAAP technique was higher than that assayed by Wright＇s stain. The difference was satistically significant. Conclusion： APAAP technique is much better than Wright＇s stain in detecting micromegakaryocytes. APAAP technique is an important method to diagnose the type of anemia.

关 键 词：贫血 诊断 小巨核细胞 APAAP 

分 类 号：R556.6[医药卫生—血液循环系统疾病]