Targeted expression of vascular endothelial growth factor 165 in the hrDNA locus mediated by hrDNA targeting vector  

作　　者：LIANG Zong-min LUO Hong YANG Yi-feng LIU Xiong-hao XIE Li XUE Zhi-gang HU Dong-xu LIANG De-sheng XIA Kun XIA Jia-hui 

机构地区：[1]Department of Cardiovascular Surgery, Second Xiangya Hospital of Central South University, Changsha 410011,China [2]National Laboratory of Medical Genetics of China, Changsha 410011,China [3]Department of Respiratory Medicine, Second Xiangya Hospital of Central South University, Changsha 410011,China

出　　处：《Chinese Medical Journal》2007年第5期427-430,共4页中华医学杂志（英文版）

基　　金：a grant from the National Natural Science Foundation of China(No.30270734)

摘　　要：Vascular endothelial growth factor （VEGF） is an angiogenic regulator that stimulates endothelial cell migration, proliferation, and angiogenesis. VEGF gene therapy is a new promising approach to induce therapeutic angiogenesis for the treatment of ischemic myocardial and limb diseases. Recently, clinical studies have demonstrated successful outcomes using plasmids, retroviruses and adenoviruses.l-3 But the safety of those vectors is poor, which has become a serious concern. Besides immunogenicity caused by viral vectors, a further problem is that viral vectors show a preference to integrate into the transcription or control region of active genes, which may induce tumors and other disorders.Vascular endothelial growth factor （VEGF） is an angiogenic regulator that stimulates endothelial cell migration, proliferation, and angiogenesis. VEGF gene therapy is a new promising approach to induce therapeutic angiogenesis for the treatment of ischemic myocardial and limb diseases. Recently, clinical studies have demonstrated successful outcomes using plasmids, retroviruses and adenoviruses.l-3 But the safety of those vectors is poor, which has become a serious concern. Besides immunogenicity caused by viral vectors, a further problem is that viral vectors show a preference to integrate into the transcription or control region of active genes, which may induce tumors and other disorders.

关 键 词：human-derived vector site-specific integration targeting expression vascular endothelial growth factor 165 

分 类 号：R373[医药卫生—病原生物学]