尿鸟苷素基因G-247A多态性与血压及水盐代谢  

Association between uroguanylin G-247A polymorphism and blood pressure/fluid and electrolytes homeostasis

作　　者：郭慧峰[1] 高平进[1] 李燕[1] 朱鼎良[1] 王继光[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院上海市高血压研究所,上海市第六人民医院老年科200025

出　　处：《中华心血管病杂志》2007年第3期233-236,共4页Chinese Journal of Cardiology

摘　　要：目的研究尿鸟苷素基因多态性与高血压及水盐代谢的关系。方法在浙江省景宁畲族自治县募集6个自然村,以核心家系为单位收集人群样本,本研究对象总计449名。采集静脉血,抽取 DNA,以限制性酶切长度多态性方法进行基因分型。留取24h 尿,检测尿量及钾、钠排泄量。使用方差分析、广义估计方程(generalized estimating equations,GEE)以及以核心家系为基础的 QTDT(quantitative transmission disequilibrium test)分析,进行关联分析。结果我们共找到10个尿鸟苷素基因变异位点,均见于 NCBI(National Centre for Biotechnology Information,www.ncbi.nlm.nib.gov)SNP数据库。我们选择 G-247A 进行基因分型。在调整协变量前以及调整性别、年龄、体重指数、吸烟、饮酒和使用抗高血压药物等变量后,尿鸟苷素基因 G-247A 基因型与血压无显著相关。但在调整协变量前后,-247AA 纯合子24 h 尿量(P=0.08)、24 h 尿钠(P=0.07)和尿钾(P<0.001)排泄量均显著增加。结论在浙江景宁人群中,尿鸟苷素基因 G-247A 基因型与水盐代谢密切相关,但该多态性与血压无显著相关。Objective To observe the association between uroguanylin G-247A polymorphism and blood pressure/fluid and dectrolytes homeostasis. Methods Uroguanylin genotype was determined by restrictive fragment length polymorphism （RFLP） and blood pressure as well as fluid and electrolytes homeostasis were measured in 442 volunteers from Jing Ning County, ZheJiang Province. Data were analyzed by ANOVA, Generalized Estimating Equations （GEE）, and Quantitative Transmission Disequilibrium Test （QTDT）. Results Ten uroguanylin gene polymorphisms were detected in 40 subjects by direct sequencing, all were reported in the NCBI SNP database. We selected the G-247A polymorphism for genotyping. Compared with G allele carriers, AA homozygotes had a higher urinary volume （P = 0. 08 ）, higher excretions of sodium （ P = 0. 07 ） and potassium （ P 〈 0. 001 ）, but similar systolic and diastolic blood pressure （P 〉 0. 32） both before and after adjustment for sex, age, body-mass index, current smoking, alcohol intake, and antihypertensive treatment. Conclusions The uroguanylin G-247A polymorphism was associated with urinary volume and sodium and potassium excretions.

关 键 词：尿鸟苷素 基因 多态性 限制性片段长度 代谢 

分 类 号：R544.1[医药卫生—心血管疾病]