原因不明复发性流产患者调节性T淋巴细胞比例及功能的测定  

The measurement of proportion and function of regulatory T cells in unexplained recurrent spontaneous abortion

作　　者：刘雨生[1] 赵淑云[1] 季静娟[1] 翟志敏[2] 童先宏[1] 骆丽华[1] 栾红兵[1] 

机构地区：[1]安徽医科大学附属省立医院生殖医学中心,合肥230001 [2]安徽医科大学附属省立医院中心实验室,合肥230001

出　　处：《中华妇产科杂志》2007年第3期176-179,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：安徽省科技攻关计划(06013125B)

摘　　要：目的探讨外周血 CD_4^+CD_(25)^+调节性 T 淋巴细胞(CD_4^+CD_(25)^+Tr 细胞)的比例和功能在原因不明复发性流产(URSA)中的作用。方法采用双荧光标记流式细胞分析技术检测15例 URSA 患者(流产组)、15例正常非妊娠妇女(正常非孕组)和13例正常妊娠妇女(正常妊娠组)外周血中CD_4^+CD_(25)^+Tr 细胞的比例。采用磁珠分选方法从3组妇女外周血淋巴细胞中分离 CD_4^+CD_(25)^+Tr 细胞和CD_4^+CD_(25)^-T 淋巴细胞,通过体外增殖抑制实验计算抑制率来评价 CD_4^+CD_(25)^+Tr 细胞对 CD_4^+CD_(25)^-T 淋巴细胞的抑制功能。结果流产组妇女外周血中 CD_4^+CD_(25)^+Tr 细胞的比例为(6.9±1.8)%,明显低于正常非孕组的(10.8±1.1)%(P<0.05)和正常妊娠组的(11.2±1.4)%(P<0.01)。流产组妇女CD_4^+CD_(25)^+Tr 细胞的抑制率为(75±6)%,明显低于正常非孕组的(89±4)%(P<0.05)和正常妊娠组的(90±4)%(P<0.01)。正常非孕组与正常妊娠组妇女 CD_4^+CD_(25)^+Tr 细胞的比例和抑制率比较,差异均无统计学意义(P>0.05)。结论 URSA 的发生可能与外周血 CD_4^+CD_(25)^+Tr 细胞的比例下降和功能降低有关。Objective To investigate the proportion and function of CD4^＋ CD25^＋ regulatory T cells （CD4^＋ CD25^＋ Tr） in unexplained recurrent spontaneous abortion （URSA）. Methods （1） Proportion measurement： the proportion of CD4^＋ CD25^＋ Tr cells in peripheral blood was measured by double-label flow cytometric analysis. The samples were taken from 15 URSA women, 15 normal non-pregnancy women and 13 normal pregnancy women. （2） Function measurement： CD4^＋ CD25^＋Tr cells and CD4^＋ CD25^- T cells were extracted from peripheral blood lymphocytes by the microbeads separation. The purity of CD4^＋ CD25^＋ Tr cells and CD4^＋ CD25^-T cells was measured by flow cytometry. The growth inhibitory effect of CD4^＋ CD25^＋ Tr cells on CD4^＋ CD25^-T cells was assessed in vitro. Results The proportion ofCD4^＋ CD25^＋ Tr cells was decreased significantly in URSA women （ 6. 9 ± 1.8 ） % than that in normal non-pregnancy women [ （ 10. 8 ± 1.1 ） % ] （P 〈 0. 05 ） and normal pregnancy women [ （ 11.2 ± 1.4 ） % ] （ P 〈 0. 01 ）. Moreover, the suppressive rate of CD4^＋ CD25^＋ Tr cells was decreased significantly in URSA women （ 75 ± 6 ） % than that in normal non- pregnancy women [ （ 89 ± 4 ） % ] （ P 〈 0. 05 ） and normal pregnancy women [ （ 90 ± 4 ） % ] （ P 〈 0. 01 ）. However, with respect to the proportion and function of CD4^＋ CD25^＋ Tr cells, there was no significant difference between normal non-pregnancy women and normal pregnancy women （ P 〉 0. 05 ）. Conclusion The results suggest that decrease in proportion and function of CD4^＋ CD25^＋ Tr cells may be associated with URSA,

关 键 词：流产 习惯性 T淋巴细胞 

分 类 号：R714.21[医药卫生—妇产科学]