药用植物刺山柑快繁再生体系的建立  

Establishment of Hypocotyl Regeneration System of Capparis spinosa L.

作　　者：栗茂腾[1] 王艳婷[1] 甘露[1] 李红[1] 付春华[1] 余龙江[1] 

机构地区：[1]华中科技大学生命科学与技术学院,武汉430074

出　　处：《华中农业大学学报》2007年第1期25-29,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金中国西部环境与生态重大研究计划(90202016)资助;岩溶动力学重点实验室开放基金资助

摘　　要：以药用植物刺山柑种子为材料,探讨了不同生长调节剂对种子萌发、芽诱导增殖和生根的影响。结果表明:利于种子萌发的启动培养基为MS附加1.0 mg/L 6-BA和2%蔗糖。从无菌苗上切取下胚轴进行不定芽诱导,其最适芽诱导增殖培养基为:MS+0.10 mg/L 6-BA+0.02 mg/L NAA,在该培养基上其增殖率可达5-6倍。利于生根的培养基为MS附加1.00mg/L IBA和100 mg/L活性炭。本研究初步建立了刺山柑组织培养及植株再生体系。In this paper the seeds of Capparis spinosa L. were taken as materials. The effects of different plant growth regulators on germination, induction, multiplication and rooting of explants were discussed. The results showed that the best medium for seed germination was MS＋ 1.0 mg/L6-BA ＋ 2% sucrose, the MS medium combined with 0. 10 mg/L6-BA and 0. 02 mg/L NAA was suitable for shoot multiplication, and the multiplication rate was about 5-6 times. The MS medium supplemented with 1.00 mg/L 6-BA and 100 mg/L activated charcoal was the best for rooting. The regeneration system of Capparis spinosa L. has been preliminarily established.

关 键 词：刺山柑 无菌苗 下胚轴 组织培养 植株再生 

分 类 号：Q813.12[生物学—生物工程] S567.239[农业科学—中草药栽培]