弥漫性脑损伤大鼠肠道防御素-5mRNA的表达  

Expression of defensin-5 mRNA in the rat intestine following diffuse brain injury

作　　者：赵希敏 刘霞 沈晓燕[3] 

机构地区：[1]上海宝山中心医院神经外科,上海201900 [2]上海宝山中心医院麻醉科,上海201900 [3]上海第二医科大学附属宝钢医院神经外科

出　　处：《中华急诊医学杂志》2007年第3期231-233,共3页Chinese Journal of Emergency Medicine

基　　金：上海市科委科研基金资助项目（034119942）

摘　　要：目的探讨弥漫性脑损伤后肠道防御素．5（RD．5）变化。方法大鼠32只，分为对照组（n=8），弥漫性脑损伤组（n=24），再分成3个亚组（24h、3d、7d）。观察回肠RD-5mRNA的表达，回肠肠黏膜的病理改变以及血液内毒素的变化。结果弥漫性脑损伤后24hRD-5mRNA的表达显著升高（P〈0.01），伤后3d降低至正常水平以下。回肠肠黏膜损伤，血液内毒素伤后24h最高（P〈0.01），3d至7d仍显著高于正常水平（P〈0.01）。结论弥漫性脑损伤早期RD-5mRNA的表达增强可能是机体的一种保护性反应。Objective To investigate the expression of rat defensin-5 （RD-5） following diffuse brain injury. Method Thirty-two SD rats were randomly divided into control group （ n = 8） and diffuse brain injury group. Rats of the diffuse brain injury group were sacrificed at 24 hours, 3 days and 7 days （ n = 8 ）. The expression of RD-5 in the rat intestine was determined with RT-PCR. Pathological changes in intestine and the endotoxin in vein blood were investigated. Results RD-5 mRNA expression in rat intestines increased significantly after diffuse brain injury at 24 hours （P 〈 0.01）, and at 3 days it was decreased to a level lower than control. Intestinal mucosal was injured. The endotoxin of vein blood in diffuse brain injury group from 24 h to 7 d was higher than that in the control group. Conclusions The increased expression of RD-5 mRNA in the early stage after the brain injury might be connected with protective response.

关 键 词：防御素-5 弥漫性脑损伤 内毒素 大鼠 

分 类 号：R686[医药卫生—骨科学]