HPLC法测定五指毛桃药材中补骨脂素和佛手柑内酯的含量  

HPLC Determination of Psoralen and Bergapten in Ficus hirta Vahl.

作　　者：钟镜金[1] 刘占强[1] 曾元儿[1] 江滨[1] 徐鸿华[1] 

机构地区：[1]广州中医药大学中药学院,广州510006

出　　处：《中药新药与临床药理》2007年第2期126-128,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：科技部重点攻关项目(2001.BA701A23-03)

摘　　要：目的建立五指毛桃药材中补骨脂素和佛手柑内酯的高效液相色谱测定方法。方法采用反相高效液相色谱法,Merck-lichrospher RP-18色谱柱(4mm×250mm,5μm),流动相:乙腈-水(35∶65),流速为1.0mLm/in,柱温:35℃;检测波长:222nm。结果补骨脂素在0.12μg～1.20μg范围内,线性关系良好;佛手柑内酯在0.03μg～0.30μg范围内,线性关系良好;补骨脂素和佛手柑内酯的平均回收率分别为100.9%和99.6%;RSD分别为2.9%和1.9%。结论首次建立了同时测定五指毛桃药材中补骨脂素和佛手柑内酯的HPLC分析方法,本方法结果准确,重现性好,可作为五指毛桃药材的质量评价方法。Objective To establish a HPLC method for the determination of psoralen and bergapten in Ficus hirta Vahl. Methods A Merck - lichrospher C18 （4mm×250mm,5μm）column was adopted. The mobile phase was acetoni- trile - water（35： 65） with the flow rate of 1.0 mL/min. The column temperature was 35 ℃, and the detection wavelength was set at 222 nm. Results Psoralen＇ s linearity was obtained in the range of 0. 12 μg - 1.20 μg（ r =0. 999 7）, and bergapten＇ s linearity in the range of 0. 03 μg - 0. 30 μg（ r = 0. 999 5）. Psoralen＇ s average recovery was 100.9 % with RSD 2.9 %, and that of bergapten was 99.6 % with RSD 1.9 %. Conclusion This is the first report of simultaneous determination of psoralen and bergapten in Ficus hirta Vahl. by HPLC, and the results showed the method is accurate, reproducible, and can serve as quality evaluation method for Ficus hirta Vahl.

关 键 词：五指毛桃 含量测定 补骨脂素 佛手柑内酯 HPLC法 

分 类 号：R284.1[医药卫生—中药学]