复方六月雪对HepG2.2.15细胞HBV DNA的抑制作用  

The inhibitory effect of Compound Liuyuexue (复方六月雪) on HBV DNA in the HepG2.2.15 cells

作　　者：张士军[1] 焦杨[1] 林兴[1] 莫国艳[1] 黄仁彬[1] 

机构地区：[1]广西医科大学药理学教研室,南宁530021

出　　处：《中药药理与临床》2007年第1期59-61,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：广西科学研究与技术开发计划项目(桂科攻0322024-5E)

摘　　要：目的:观察复方六月雪体外抗乙型肝炎病毒(HBV)的作用。方法:采用MTT法检测复方六月雪对HepG2.2.15细胞的半数毒性浓度(TC50)和最大无毒浓度(TC0);在最大无毒浓度(TC0)基础上观察不同浓度药物作用于HepG2.2.15细胞,分别在第72h和144h收集细胞培养上清液,采用实时荧光定量PCR法检测上清液HBV DNA的含量。结果:TC50为3.070mg/ml,TC0为0.945mg/ml,复方六月雪对HepG2.2.15细胞毒性较低。无毒浓度下的复方六月雪在HepG2.2.15细胞培养中可有效地抑制细胞HBVDNA的复制。结论:CLYX在体外有显著的抗HBV的作用,且毒性较低。Objective： To study the inhibitory effect of traditional Chinese medicine Compound Liuyuexue （CLYX） on HBV in vitro. Methods ： The TC50 and TC0 of CLYX to the HepG2.2.15 cells were determined by MTT assay. At non-cellulotoxic concentration, the different concentrations of CLYX were added respectively to HepG2.2.15 cells, which were incubated subsequently. At 72h and 144h after the treatment with drugs, the culture media were collected for determining the quantity of HBV DNA detected by real time fluorescence quantitative PCR （FQ-PCR）. Results ：TCso =3. 070mg/ml, TC0 =0. 945mg/ml. CLYX had little cellulotoxicity. CLYX at the largest non-cellulotoxic concentration could decrease the HBV DNA level in HepG2.2.15 cell. Conclusion： CLYX can inhibit significantly HBV in vitro, and is a lower toxic drug.

关 键 词：复方六月雪 抗乙型肝炎病毒 HBV DNA HEPG2.2.15细胞 荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]