用于移植气管局部的表皮生长因子明胶微球的研究  

Research on epidermal growth factor gelatin microspheres for topical application in tracheal transplantations

作　　者：赵晋波[1] 李小飞[1] 吴红[2] 刘锟[1] 梁志波[1] 程庆书[1] 

机构地区：[1]第四军医大学唐都医院胸外科,陕西西安710038 [2]第四军医大学药学系化学教研室,陕西西安710032

出　　处：《生物医学工程与临床》2007年第2期81-84,共4页Biomedical Engineering and Clinical Medicine

基　　金：国家自然科学基金（39970707）;陕西省自然科学基金（99SM37）

摘　　要：目的制备可用于移植气管局部的表皮生长因子（epidermal growth factor，EGF）明胶微球（gelatin microspheres，GMs），研究其生物学活性。方法采用改良双相乳化冷凝法制备表皮生长因子明胶微球（epidermal growth factor gelatin microspheres，EGF-GMs），计算其溶胀率，观测微球分散度、粒径及外观形态。采用BALB／c3T3作为效应细胞，分为3组，A组（EGF-GMs组）、B组（游离EGF组）、C组（空白GMs组），进行细胞计数并应用流式细胞仪观测细胞周期评估表皮生长因子明胶微球生物学活性。结果制得的明胶微球大小均匀，平均粒径为107μm。细胞计数：第1、3天时。B组＞A组＞C组，且差异有统计学意义（P＜0．05）；第7天时，A、B组高于C组，差异有统计学意义（P＜0．05），但A、B两组间无统计学意义（P＞0．05）；第11天时，A组＞B组＞C组，且差异有统计学意义（P＜0．05）。第11天，流式细胞分析结果：A组增殖指数和S期细胞比例均大于B、C组，三组间差异有统计学意义（P＜0．05）。结论表皮生长因子明胶微球粒径大小适合局部应用，能够在较长时间缓释具有生物学活性的表皮生长因子。Objective To study biologic activity of the epidermal growth factor gelatin microspheres （EGF-GMs） for topical application in tracheal grafts. Methods The EGF-GMs were prepared by optimal double-phase emulsified condensation polymerization. The swelling ratio, dispersity, particle diameter and appearance of the GMs were observed. The BALB/c 3T3 cells were divided into three groups according to the different ingredients added into the DMEM culture medium ie EGF-GMs（group A） ,EGF（group B） and GMs（group C）. The proliferation of the BALB/c 3T3 cells was measured by cell counting method and flow cytomerty. Results The gelatin microspheres were predominant in quality, even and uniform spheres with mean particle size of 107 μm. The in vitro cellular study showed that on the first day and third day after plate culture ,the cell number in group B was larger than those in group A and group C （P 〈 0.05） ; The cell number in group A and group B were larger than that in group C （P 〈 0.05 ） in seventh day, but there was no significant difference between group A and group B （P 〉 0.05 ） ;The cell number in group A was larger than those in group B and group C （P 〈 0.05） in eleventh day. The flow cytometry revealed that the proliferation index and S-phase fraction in group A were the highest among the three groups （P 〈 0.05）. Conclusion The particle size of EGF-GMs was suitable for topical application in trachea transplantation and the EGF-GMs could release active EGF for a long period.

关 键 词：表皮生长因子 局部应用 明胶微球 缓释 

分 类 号：R943[医药卫生—药剂学]