人主动脉壁脱细胞血管基质的制备  

Study on Cell Extraction from Human Aortas

作　　者：于海明[1] 冯德广[1] 李红卫[1] 余海彬[1] 徐成阳[1] 林彬 

机构地区：[1]郑州大学第二附属医院心血管外科

出　　处：《医药论坛杂志》2007年第1期11-13,共3页Journal of Medical Forum

摘　　要：目的探讨应用同种去细胞主动脉血管壁支架体外构建组织工程管道的可行性。方法取液氮保存的同种主动脉带瓣管道。首先置于Tris低渗缓冲液室温下溶解血管壁细胞,血管壁用D-Hanks液充分清洗后,再用含十二烷基硫酸钠(SDS)的Tris低渗缓冲液室温下再孵育24h,洗涤脱去细胞;最后用Tris等渗缓冲液充分冲洗残留细胞,完成脱细胞支架制备。并对脱细胞后的管壁进行光镜及电镜形态学观察以及进行理化性能测定。结果人主动脉血管壁经SDS法去细胞后,经光镜和电镜观察,脱细胞后血管壁无细胞残留,胶原纤维和弹性纤维保留完整,形态学无明显变化;生物学性能也没有明显改变。结论SDS法去细胞效果良好,初步制造了同种主动脉血管壁脱细胞基质材料,为构建同种带瓣管道提供了较合适的材料。Objective To investigate a method to remove cellular components from human aortas, resulting scaffolds of acellular tissue matrix （ACTM） for tissue engneering of vessel. Methods The active homograft bioprosthetic pediculated conduit preserved in liquid nitrogen were selected as experinaental material and decellularized by sodium dodecyl sulplate （SDS） containing low osmotic Tris buffer. The morphological integrity of acellular scaffold was investigated by means of inverted phased contrast microscope and scanning electron microscope. The shrinkage temperature , thickness and water content were compared between active aortic wall and acellular wall. Results Light and electron microscopy confirmed that all the cellular constituents were removed without ultrastructural damage to fibrous component. Water content in decellular aortic wall was increased significant; thickness and shrinkage temperature of the decellularized human ho aortic had no significant differences. Conclusion Human aortic can be almost completely acelluarized by SDS containing low osmotic Tris buffer procedure without imparing the mechanical propertiy of the tissue. This kind of acellular tissue could be used to develop tissue engineering for ho aortic conduit substitute.

关 键 词：主动脉 去细胞基质 血管 同种带瓣管道 

分 类 号：R446.5[医药卫生—诊断学]