NP30抗/体检测试剂盒在云南大山区型血吸虫病流行区的现场应用  

Field application of NP30/antibody detection kit to detecting schistosomiasis in mountainous endemic regions in Yunnan

作　　者：吴玉龙[1] 程梅[2] 李红星[1] 孟玮[1] 

机构地区：[1]滨州医学院病原生物学教研室,滨州市256603 [2]南京医科大学生物化学与分子生物学系

出　　处：《滨州医学院学报》2007年第1期13-15,共3页Journal of Binzhou Medical University

摘　　要：目的现场评价NP30抗/体检测试剂盒在大山区型血吸虫病流行区筛查病人中的应用价值。方法在云南省大山区型血吸虫病流行区,选择3个自然村作为调查现场,随机抽取10-70岁的村民作为调查对象,进行血吸虫病病史个案调查;收集粪便标本作病原学检查;静脉采血,分离血清,分别采用NP30抗/体检测试剂盒和酶联免疫吸附试验试剂盒(SEA/ELUSA法)进行血吸虫病免疫学诊断。结果3个村共筛查村民506人,粪便孵化法检测阳性164例,阳性率为32.41%;NP30和SEA/ELISA的阳性符合率分别为58.3%和75.0%;在164例粪检阳性血清样本中,NP30和SEA/ELISA的阳性符合率分别为87.88%和84.71%。在342例粪孵阴性血清样本中,NP30和SEA/ELISA的检出率分别为44.15%和70.47%。结论NP30/抗体检测试剂盒在大山区型血吸虫病流行区现场大规模筛查病人中具有较高的敏感性和特异性。Objective To evaluate the field application value of the kit of NP30/andtibody detection in screening schistosomiasis in mountainous endemic regions. Methods In a mountainous endemic region of schistosomiasis in Yunnan Province, random samples of the villagers aged 10-70 years from three natural villages were examined by using stool hatching test for parasitological examination, and the sera from the villages were tested with the kits of NP30/and SEA/ELISA for immune examination respectively. Results A random sample of 506 villagers from three natural villages were screened, the positive rates of stool hatching test, NP30/antibody detection and SEA/ELISA were 32. 41% ,58.3% and 75.0% respectively. The positive coincidence rates of NP30/and SEA/ELISA were 87. 88% and 84. 71% in 164 stool positive subjects. The detection rates of NP30/and SEA/ELISA were 44. 15% and 70. 47% in 342 stool negative subjects. Conclusion The NP30/antibody detection kit has a high sensitivity and good specificity for screening patients infected with Schistosoma japonicum on large scale in mountainous endemic regions. The assay is fast and easy to be done and available for application in the endemic field.

关 键 词：血吸虫病 NP30/抗体检测试剂盒 大山区型血吸虫病流行区 现场应用 

分 类 号：R383.2[医药卫生—医学寄生虫学]