一种白蛋白纳米粒的制备与评价  

Studies on Preparation and Evaluation of Albumin Nanoparticles

作　　者：张良珂[1] 侯世祥[2] 宋相容[2] 卢懿[2] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]四川大学华西药学院药剂教研室,成都610041

出　　处：《中国药学杂志》2007年第5期365-367,共3页Chinese Pharmaceutical Journal

基　　金：教育部高等学校博士学科点专项科研基金资助(20020610092);重庆医科大学科研基金(B10001)

摘　　要：目的研究白蛋白纳米粒的去溶剂化法制备工艺。方法采用乙醇作为非溶剂,考察了白蛋白质量浓度和pH值、非溶剂(乙醇)体积和加入速度、交联剂体积等对纳米粒产率、粒径以及游离氨基含量的影响。结果将白蛋白100 mg溶于碳酸盐缓冲液(pH9)10 mL中,搅拌下按1.0 mL.min-1的速度滴加乙醇60 mL,加入0.025 kg.L-1戊二醛溶液50μL,25℃固化12h,减压浓缩除去乙醇,得白蛋白纳米粒胶体。结论确定了去溶剂化-交联法制备白蛋白纳米粒的优化工艺。OBJECTIVE To optimize the preparation method of albumin nanoparticles by desolvation. METHODS Nanoparticles were prepared by desolvation and subsequent crosslinking of albumin. The effects of several factors such as albumin concentration, crosslinking time,the volume of non-solvent and erosslinking agent,the pH value of the buffer solution and the dripping rate of non-solvent on the nanopartieles yield,diameters and free amino group（FAG） content of the products were examined respectively. RESULTS Albumin（ 100 mg） was dissolved in 10 mL of NaHCO3/Na2CO3 buffer. Under constant stirring,desolvation was achieved by dropwise addition of 60 mL ethanol. After the desolvation process,50 μL of 2.5% glutaraldehyde solution was added to induce particle crosslinking. The crosslinking process was performed by stirring the suspension for 12 h. The ethanol was evaporated and albumin nanoparticle suspension was acquired. CONCLUSION The optimal preparation of albumin nanoparticle is established.

关 键 词：白蛋白 纳米粒 去溶剂化 制备 

分 类 号：R944.9[医药卫生—药剂学]