双向电泳-质谱技术筛选矽肺血清差异蛋白的研究  

Utilizing 2-DE and MALDI-TOF MS/MS to screen differentially expressed serum proteins of silicosis

作　　者：曾家伟[1] 王世鑫[2] 赵学峰[1] 魏茂提[2] 涂植光[1] 

机构地区：[1]重庆医科大学医学检验系,重庆403016 [2]中国人民武装警察部队医学院科研部,天津300162

出　　处：《中华劳动卫生职业病杂志》2007年第3期136-141,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：天津市科技攻关培育项目（033106011）（本实验中双向电泳和质谱鉴定在上海复旦大学蛋白质组学实验室完成,该实验室老师和伯乐中国公司工程师给予技术支持,志谢）

摘　　要：目的建立正常人和矽肺人群血清双向电泳图谱，寻找矽肺血清差异蛋白，进一步探索其发病机制。方法采用双向凝胶电泳（2-dimensional gel eleetrophoresis,2-DE）和基质辅助激光解吸飞行时间串联质谱（matrix—assisted laser desorption／ionization time-of-flight tandem mass spectrometry．MALDI-TOF-MS／MS）对矽尘接触人群和对照组人群血清进行比较蛋白质组学研究，寻找矽肺血清差异蛋白。结果初步发现了补体C4、富含亮氨酸的α-2糖蛋白、α-1抗胰蛋白酶在0^＋组血清中表达明显升高，差异有统计学意义（P〈0．01）；在对照组、矽尘接触组、I期矽肺组中表达均较低。转铁蛋白在0^＋血清中表达明显下调，差异有统计学意义（P〈0．01）。血浆谷胱甘肽过氧化物酶、四连接素、载脂蛋白A-I，甲状腺素运载蛋白在对照组人群和矽尘接触组血清中表达相当，在0^＋和I期矽肺组中呈下降趋势。结论初步发现补体C4、富含亮氨酸的α-2糖蛋白、α-1抗胰蛋白酶、转铁蛋白、血浆谷胱甘肽过氧化物酶、四连接素、载脂蛋白A-I和甲状腺素运载蛋白在矽尘接触组、0^＋组和I期矽肺组人群血清中存在差异表达，但结果有待运用其他生物学的方法进行具体的验证并探索其机制。Objective To establish 2-dimensional gel electrophoresis （2-DE） images and seek differentially expressed serum proteins for understanding the pathogenesis of silicosis. Methods 2-DE and matrixassisted laser desorption/ionization time of flight tandem mass spectrometry （MALDI-TOF-MS/MS） were used to screen differentially expressed serum proteins among silica-exposed population, suspect of silicosis （0^＋）, phase one （I） group with silicosis and control group（non silica exposure）. Results Complement C4, leucinerich alpha-2-glycoprotein and alpha-l-antitrypsin were significantly highly expressed in suspect of silicosis （0^＋） group（P〈0.01）, but lowly in other groups. Inversely, serotransferrin was significantly down-regulated only in suspect of silicosis （0^＋） group （P〈0.01）. Plasma glutathione peroxidase, tetranectin, apolipoprotein A-I and transthyretin were equally expressed in the serum of control group and silica-exposed population group, but decreased in the suspect of silicosis （0^＋） and phase （I） group. Conclusion Complement C4, leucine-rich alpha-2- glyeoprotein, alpha-l-antitrypsin, serotransferrin, plasma glutathione peroxidase, tetranectin, apolipoprotein A- I and transthyretin are differentially expressed in the silica-exposed group and phase （I） group with silicosis, and the result shoud be validated by other biochemical technologies.

关 键 词：矽肺 血清 蛋白质组学 生物标记物 

分 类 号：R686[医药卫生—骨科学]