多溴联苯醚对SH-SY5Y细胞氧化应激及凋亡的影响  

Effects of PBDE-47 on oxidative stress and apoptosis in SH-SY5Y cell

作　　者：张明[1] 何卫红[2] 何平[2] 夏涛[2] 陈学敏[2] 王爱国[2] 

机构地区：[1]天津市疾病预防控制中心,300011 [2]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系教育部环境与健康重点实验室

出　　处：《中华劳动卫生职业病杂志》2007年第3期145-147,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金重大项目（45090393）

摘　　要：目的观察2，2’，4，4’-四溴联苯醚（PBDE-47）对人神经母细胞瘤SH-SY5Y细胞株氧化应激和凋亡的影响及活性氧在细胞凋亡中的作用。方法实验共分4组，PDDE-47终浓度为0、2、4、8μg／ml，分别作为对照组，低、中、高剂量组。用2、4、8μg／mlPBDE-47对培养的SH-SY5Y细胞染毒，24h后检测细胞存活率、活性氧（ROS）水平、丙二醛（MDA）含量及凋亡率。结果与对照组比较，低剂量组细胞存活率略有上升，中、高剂量组细胞存活率明显降低，差异有统计学意义（P〈0．05）；MDA含量随染毒剂量的增加呈上升趋势，中、高剂量组MDA含量明显高于对照组差异有统计学意义（P〈0．05）；各染毒剂量组ROS水平明显高于对照组，差异有统计学意义（P〈0．05）；中、高剂量组细胞凋亡率与对照组之间的差异有统计学意义（P〈0．05）。结论PBDE-47可引起SH-SY5Y细胞氧化应激及凋亡，活性氧可能在PBDE-47诱导的凋亡中起重要作用。Objective To investigate the oxidative stress and apoptosis induced by 2, 2＇, 4, 4＇-polybrominated diphenyl ethers （PBDE-47） in human neuroblastoma SH-SY5Y cells, and to explore the involved role of reactive oxygen species （ROS） on apoptosis. Methods The rate of cellular survivors, intracellular ROS level, malondialdehyde （MDA） content and percentage of apoptosis were measured respectively after the SHSY5Y cell were exposed to 2, 4, 8 μg/ml PBDE-47 for 24 h in vitro. Results The rate of cellular survivors in the low dose PBDE-47-treated group was higher than the control group （P〈0.05）, but the moderate and high dose PBDE-47-treated groups were significantly lower than the control group （P〈0.05）. The MDA contents in the moderate and high dose PBDE-47-treated groups were significantly higher than the control group （P〈0.05） and increased with increase of PBDE-47 exposure concentrations. Compared with the control group, the levels of ROS were significantly increased with increase of PBDE-47 concentrations （P〈0.05）. In the moderate and high dose PBDE-47-treated groups, the percentages of apoptosis were significantly higher than that of the control group （P〈0.05）. Conclusion PBDE-47 can induce oxidative stress and apoptosis in SH-SY5Y cell. ROS may play an important role in the apoptosis induced by PBDE-47.

关 键 词：多溴联苯醚 SH-SY5Y细胞 氧化性应激 细胞凋亡 

分 类 号：R686[医药卫生—骨科学]