川芎嗪对HaCaT细胞中成纤维细胞生长因子10影响的实验研究  

Experimenatal Study on the Effect of Fibroblast Growth Factor 10 (FGF10) on HaCaT Cells with Tetramethylpyrazine

作　　者：于春水[1] 谭升顺[1] 眭维耻[2] 袁景奕[1] 汪淼芹[2] 郗彦萍[1] 

机构地区：[1]西安交通大学第二附属医院皮肤科,陕西西安710004 [2]川北医学院附属医院皮肤科,四川南充637000

出　　处：《中国皮肤性病学杂志》2007年第3期141-143,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的研究川芎嗪对HaCaT细胞中FGF10mRNA转录和FGF10蛋白翻译的影响。方法HaCaT细胞以不同浓度的川芎嗪处理48h后,用原位杂交和免疫荧光法检测FGF10mRNA转录和FGF10蛋白翻译的改变情况。结果与对照组比较,处理48h后,FGF10的mRNA水平和蛋白水平均减少,随着川芎嗪剂量的增加,FGF10mRNA转录和FGF10蛋白翻译均减少,两组间比较,有显著性差异(P<0.01)。结论川芎嗪可抑制HaCaT细胞中FGF10mRNA的转录及其蛋白的翻译,随着川芎嗪剂量的增加,HaCaT细胞中FGF10的转录和翻译均减少。Objective To further illuminate the effect of the transcription of FGF10 mRNA and the translation of FGF10 protein in cultured HaCaT cells with tetramethylpyrazine treated. Methods HaCaT cells were treated with different concentration of tetramethylpyrazine for 48 h, then the transcription of FGF10 mRNA and the translation of FGF10 protein in these cells were detected by immunofluorescence and in situ hybridization assay. Results Compared with the control groups,with the concentration of tetramethylpyrazine increased,the transcription of FGF10 mRNA and the translation of FGF10 protein levels were deceased. There was significant differences among different groups （P 〈 0.01 ）. Conclusion Tetramethylpyrazine could inhibit the transcription of FGF10 mRNA and the translation of FGF10 protein in HaCaT cells, with the dose of tetramethylpyrazine increased, the stains of both FGF10 mRNA and FGF10 protein in HaCaT cells reduced.

关 键 词：川芎嗪 HACAT细胞 成纤维细胞生长因子10 

分 类 号：R285.6[医药卫生—中药学]