艾灸对实验性RA家兔滑膜细胞MAPK信号通路影响的研究  

The Influence on MAPK Signal Pathway in RA Synovial Cell of Experimental Rabbits by Moxibustion

作　　者：杨馨[1] 杨慎峭[1] 周海燕[1] 余曙光[1] 冷为军[1] 刘旭光[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《中华中医药学刊》2007年第3期470-474,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金重大研究计划资助(90209022)

摘　　要：目的:观察艾灸对实验性RA家兔滑膜细胞MAPK信号通路的影响,揭示艾灸治疗实验性RA的分子信号机制。方法:运用基因芯片及生物信息分析技术检测分析MAPK信号通路中96种相关信号分子的表达。结果:造模组与正常对照组相比较,MAPK通路异常激活,Cyclin A1、p38 MAPK、ERK1、JNK1、N-ras等相关信号分子表达上调;艾灸组与造模组相比较,MAPK通路中异常激活的Cyclin A1、p38 MAPK、ERK1、JNK1、N-ras等相关信号分子的表达明显下调。结论:艾灸对实验性RA滑膜细胞MAPKs信号通路的异常激活有明显抑制作用。Objective： Observe the influence of MAPK pathway＇s signal transduction in RA synovial cell of experimental rabbits by moxibustion, and reveal the molecular signal mechanism of experimental RA by moxibustion. Methods ： Apply gene chip inspection and bioinformation analytical technique to detect the 96 sorts of signal molecule of MAPK pathway. Results： Comparing model group with normal group, MAPK pathway has been activated abnormally,and as a result, the expression of signal molecule such as Cyclin A1 ,p38 MAPK,ERK1 ,JNK1 ,N - ras has been up - regulated; comparing moxibustion group with model group, the expression of signal molecule such as Cyclin A1 ,p38 MAPK,ERK1 ,JNK1, N - ras has been down - regulated after therapy. Conclusion ： Moxibustion plays a very important roll of transparent inhibition in abnormal activation of MAPK pathway＇s signal transduction in RA synovial cell of experimental rabbits.

关 键 词：艾灸 类风湿性关节炎 滑膜细胞 基因芯片 MAPK通路 

分 类 号：R245.81[医药卫生—针灸推拿学]