大黄中主要成分清除超氧阴离子自由基的ESR研究  

ESR Study on the Scavenging Effects of the Main Ingredients in Rheum Palmatum L. on Superoxide Anion Radical

作　　者：罗志毅 黄新[2] 包国荣[3] 

机构地区：[1]漳州片仔癀药业股份有限公司技术中心,福建漳州363000 [2]中国科学院福建物质结构研究所,福建福州350001 [3]福建中医学院药学系,福建福州350003

出　　处：《中华中医药学刊》2007年第3期612-614,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:通过研究掌叶大黄中主要成分大黄酸、芦荟大黄素、大黄素、大黄素甲醚和大黄酚清除超氧阴离子自由基(O2.-)的作用,从分子生物学的角度探讨大黄延缓衰老作用的可能机理。方法:采用电子自旋共振技术(ESR)分别检测大黄中主要成分对O2.-的清除作用。结果:大黄中主要成分对O2.-的清除作用,依次为大黄酸>大黄酚>大黄素>大黄素甲醚>芦荟大黄素。结论:大黄所具有的延缓衰老的作用,可能与其所含的主要成分大黄酸、芦荟大黄素、大黄素、大黄素甲醚和大黄酚具有清除O2.-的能力有关。Objective： To study the scavenging effects of Rhein, Aloe - emodin, Emodin, Physcion and Chrysophanol which were the main ingredients in Rheum palmatum L. on superoxide anion radical and to investigate the mechanism of Rheum palmatum L. in retarding aging at molecular biology lever. Methods： The electron spin resonance （ESR） was used to observe the scavenging effect on the superoxide anion radical（ O2^- ）of the main ingredients in Rheum palmatum L. Results： All of the Rhein ,Aloe-emodin,Emodin,Physcion and Chrysophanol were effective in scavenging O2^- produced in the alkaline DMSO system,and the ability was Rhein 〉 Chrysophanol 〉 Emodin 〉 Physcion 〉 Aloe - emodin. Conclusion ： Rheum palmatum L. has the effect of retarding aging. Perhaps this is relevant to the scavenging effects on the superoxide anion radical（ O2^- ）of the main ingredients in the herb.

关 键 词：大黄 药理学 抗氧化剂 自由基清除剂 衰老 药物作用 电子自旋共振(ESR) 

分 类 号：R285.5[医药卫生—中药学]