酮体外抗氧化作用研究  

Antioxidative effect of xanthones in Vitro

作　　者：张媛媛[1] 管棣[1] 谢青兰[1] 余德河[1] 

机构地区：[1]西南交通大学生物工程学院,四川成都610031

出　　处：《中成药》2007年第3期342-345,共4页Chinese Traditional Patent Medicine

摘　　要：目的:研究3种来自大籽獐牙菜的酮类化合物1,8-二羟基-3,7-二甲氧基酮(methylswertianin,MET)、1,5,8-三羟基-3-甲氧基酮(bellidifodin,BEL)和1-羟基-3,7,8-三甲氧基酮(decussatin,DEC)的抗氧化作用。方法:采用H2O2和VC-Fe2+两种羟自由基发生系统;硫代巴比妥酸法测定大鼠肝匀浆及肝线粒体中脂质过氧化物丙二醛(MDA)含量;分光光度法测定大鼠红细胞氧化溶血和肝线粒体膨胀程度研究酮的抗氧化效果。结果:3种酮均能有效抑制MDA的产生,减少大鼠红细胞氧化性溶血,减轻肝线粒体膨胀程度;其中BEL效果最好,其次是MET和DEC。结论:酮具有明显的抗脂质过氧化作用。AIM： To study the antioxidative effect of three kinds of xanthone ：methylswertianin（ MET）, bellidifodin（BEL） and decussatin （DEC） extracted from Swertia macrosperma C.B. clark. METHODS： Hydroxy radical was generated from H2O2 and VC-Fe^2＋ system; MDA contents in liver homogenate and mitochondria were measured by thiobabituric acid assay; the hemolysis of RBC and the swelling extent of mitochondria were detected by spectrophotometric methods. RESULTS： Xanthones could inhibit the generation of MDA, the hemolysis of RBC, and the swelling of mitochondria. The inhibitory effect of BEL was stronger than that of MET and DEC. CONCLUSION： Xanthones possess a good antioxidant activity.

关 键 词：[口山]酮 大籽獐牙菜 脂质过氧化 羟自由基 

分 类 号：R285.6[医药卫生—中药学]