鸭瘟病毒部分基因的克隆与序列分析  

Partial cloning and sequene analysis of duck plague virus genomic DNA

作　　者：李玉峰[1] 欧阳文军[2] 周秋平[3] 黄兵[2] 马秀丽[2] 宋敏训[2] 杨汉春[1] 

机构地区：[1]中国农业大学动物医学院农业部预防兽医学重点开放实验室,北京海淀100094 [2]山东省农业科学院家禽研究所禽病诊断与免疫重点实验室,山东济南250023 [3]海南省畜牧兽医管理站,海南海口570203

出　　处：《中国兽医杂志》2007年第3期5-7,共3页Chinese Journal of Veterinary Medicine

摘　　要：用BamH Ⅰ、Eco RⅠ、Hind Ⅲ、Pst Ⅰ、SalⅡ5种限制性内切酶消化鸭瘟病毒基因组DNA，回收2～6kb片段连入pUC19载体，构建了鸭瘟病毒基因组部分文库。选取部分克隆进行序列测定，获得5个UL片段基因，分别为UL33、UL34、UL45、UL46和UL47。序列比较显示，它们与α-亚科疱疹病毒其他成员相应基因具有不同程度的同源性。这是国内外对鸭瘟病毒以上基因序列的首次报道。Duck plague virus（DPV） genomic DNA was digested by restriction endonucleases BamH Ⅰ ,EcoR Ⅰ ,Hind Ⅲ ,Pst Ⅰ and Sal Ⅰ . 2-6 kb DNA fragments were extracted and inserted into vector pUC19 to construct DPV genomic library. Some of the clones were sequenced and five new genes were found which were named UL33, UL34, UL45, UL46 and UL47. They showed homology to the corresponding genes of the members of Alphaherpesvirinae by sequence alignments on a certain extent. This is the first report of the DPV genes above.

关 键 词：鸭瘟病毒 基因组文库 基因克隆 序列分析 

分 类 号：S858.32[农业科学—临床兽医学]