人骨髓基质干细胞成骨诱导后端粒酶活性的检测分析  

The Analysis of Telomerase Activity of Human BMSCs

作　　者：周恒[1] 李宇琳[1] 孙剑[1] 刘广鹏[1] 

机构地区：[1]上海组织工程研究与开发中心组织工程(上海)国家工程研究中心,上海200235

出　　处：《组织工程与重建外科杂志》2007年第1期33-35,共3页Journal of Tissue Engineering and Reconstructive Surgery

摘　　要：目的分析人骨髓基质干细胞作为组织工程种子细胞,在长期体外扩增并进行成骨诱导下,其端粒酶活性是否发生异常的改变。方法采用端粒酶活性PCR-ELISA检测法分析3例人骨髓基质干细胞成骨诱导样本。结果人骨髓基质干细胞体外成骨诱导培养至第6代,其端粒酶活性未出现异常增高。结论人骨髓基质干细胞在体外长期成骨诱导培养扩增的条件下,端粒酶活性未出现异常改变。Objective To analyze the telomerase activity of human BMSCs which are used as seed cells for tissue engineering during muhigeneration culture. Methods Roche＇s telomerase PCR-ELISA kit was used to detect the telomerase activity of human BMSCs. Result No abnormal change was detected in the telomerase activity test of human BMSCs after muhigeneration culture till P6 in vitro. Conclusion The telomerase activity of human BMSCs measured up to the security prescript after muhigeneration culture.

关 键 词：骨髓基质干细胞 端粒酶活性 

分 类 号：R813.12[医药卫生—放射医学]