吸烟对骨折愈合影响过程中TNF-α的表达变化及意义  

Effects and significance of cigarette smoking on the expression of TNF-αduring fracture healing

作　　者：王勇平[1] 陈根元[1] 侯卫华[1] 蒲兴海[1] 萧文耀[1] 胡龙[1] 杨成业[1] 聂富祥[1] 

机构地区：[1]兰州大学第一医院骨科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2007年第1期23-25,共3页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的研究TNF-α在吸烟对骨折愈合影响过程中的表达水平,探讨吸烟与骨折愈合的关系及其可能机理。方法将80只雄性Wistar大白鼠随机分成对照组(不吸烟)、被动吸烟组(按吸烟时间及吸烟量分为2组:轻度吸烟组和重度吸烟组)、吸烟戒烟组共4组;制作大鼠骨折模型后用一定吸烟量对实验组大鼠进行干预,分别于术后1、7、14、21、28 d取各组大鼠血浆,检测血浆TNF-α水平。结果术前1 d,3个实验组大鼠血浆TNF-α水平与对照组比较,差异无显著性(P＞0．05);术后28 d,3个实验组大鼠血浆TNF-α水平均显著高于对照组(P＜0．05),随吸烟时间延长,同组间TNF-α水平逐渐升高。结论吸烟是骨折愈合的不利因素,吸烟升高血浆TNF-α水平可能是吸烟刺激骨吸收,使大鼠骨吸收增强,导致骨折延迟愈合。Objective To study the expression of TNF-α in cigarette smoking rats during fracture healing and investigate the relationship between cigarette smoking and fracture healing. Methods Animal models of fracture rats were established and 80 male Wistar rats were randomly grouped into normal control, anti-smoking, short-term and long-term smoking. Radioimmunoassay technique was used to detect the expression of TNF-α in rat blood. Results Compared with normal control group, the expression of TNF-α was increased significantly in smoking group （P 〈0.05）. Conclusions Cigarette smoking is one of the disadvantage factors during fracture healing. Cigarette smoking increases the expression of TNF-α in rat blood, which raises the absorption of the bone and delays the healing of fracture.

关 键 词：被动吸烟 骨折愈合 TNF-Α 大鼠 

分 类 号：R683[医药卫生—骨科学]