外周血Th1/Th2淋巴细胞失衡与2型糖尿病肾病的关系  

Relationship between Th1/Th2 lymphocyte imbalance in peripheral blood and type 2 diabetic nephropathy

作　　者：吴晓燕[1] 汤旭磊[1] 高林[1] 陈莉[1] 成建国[1] 

机构地区：[1]兰州大学第一医院内分泌科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2007年第1期30-33,共4页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的通过检测2型糖尿病肾病患者外周血CD4^+辅助淋巴细胞分泌的细胞因子,探讨外周血Th1/Th2淋巴细胞失衡与2型糖尿病肾病的关系。方法运用三色荧光标记法流式细胞术检测外周血中Th1/Th2淋巴细胞。结果2型糖尿病无肾病者Th1数、Th1/Th2比值与正常对照组相比明显降低(P＜0．01),2型糖尿病早期肾病患者Th1数、Th1/Th2比值明显高于正常对照组(P＜0．01),2型糖尿病临床肾病者Th1数,Th1/Th2比值较正常对照组降低,但无显著差异。2型糖尿病各组Th2数与正常对照组无明显差异。2型糖尿病患者外周血Th1淋巴细胞和HbA1c呈负相关关系(r=-0．688,P＜0．01)。结论2型糖尿病患者存在Th1/Th2淋巴细胞的失衡,在其肾脏病变发病的不同阶段,存在不同的Th1和Th2优劣势,细胞和体液免疫在肾病发生的不同阶段发挥了不同的作用。Objective To investigate the relationship between Th1/Th2 lymphocyte imbalance in peripheral blood and type 2 diabetic nephropathy. Methods The distributions of Th1/Th2 cells were determined by 3-colored fluorescence labeled flow cytometry in diabetic nephropathy （DN） groups and the controls. Results Thl cells and Th1/Th2 ratios were significantly reduced in diabetic patients when compared with the controls （P 〈0.05）. Thl cells and Th1/Th2 ratios were significantly increased in type 2 diabetic patients with microalbuminuria when compared with normal controls （P 〈0.01）. There were no difference in the Th1/Th2 ratios and Thl cells between diabetic patients with macroalbuminuria and the controls. Thl cells were negatively correlated with HbA1c （r = -0.688, P 〈0.01）. Conclusion The results show that Th1/Th2 imbalance exists in peripheral blood of patients with type 2 diabetic nephropathy. Th cells may play an important role in the occurrence and development of DN.

关 键 词：2型糖尿病肾病 TH细胞 流式细胞术 

分 类 号：R392.6[医药卫生—免疫学]