检测DR5双抗体夹心ELISA方法的建立及应用  

Establishment of quantitative assay DR5 double antibodies sandwich ELISA

作　　者：白慧玲[1,2] 刘广超[1,2] 赵粤萍[1,2] 马远方[1,2] 

机构地区：[1]河南大学免疫学研究所,河南开封475001 [2]河南大学细胞与分子免疫学实验室,河南开封475001

出　　处：《河南大学学报（医学版）》2007年第1期21-24,共4页Journal of Henan University:Medical Science

基　　金：河南省杰出人才创新基金资助项目(0321001800);河南省医学科技创新人才基金资助项目(2002119)

摘　　要：目的:建立检测DR5双抗体夹心ELISA方法,通过检测慢性乙型肝炎患者血清可溶性DR5水平,探讨其在肝炎发病机理中的作用。方法:采用本室制备的鼠抗DR5的单抗YM366ED作为包被抗体,鼠抗DR5的单抗YM366EC标记HRP,建立检测sDR5的ELISA,并测定52例慢性乙型肝炎和30例正常人血清sDR5浓度。结果:建立了检测人sDR5的夹心ELISA法,方法的变异系数小于5%,回收率85%以上。慢性乙型肝炎患者血清sDR5浓度明显高于正常对照组。结论:成功地建立了一种可用于检测血清sDR5的双抗体夹心ELISA,为判定某些相关疾病患者的病情、疗效及预后提供有用的工具。血中sDR5在慢性乙型肝炎发病过程中可能起着重要作用,检测病人血中sDR5水平可作为慢性乙型肝炎疾病活动性指标。Objective： To establish quantitative assay DR5 double antibodies sandwich ELISA with sensitivity. specificity, accuracy and repetition. Methods： sDR5 serum concentration of 52 case of ehronic hepatitis B and 30 healthy persons were detected with monoclonal antibody in rat＇s antibody as coated plate, which was made by our lab, HRP was marked by monoelonal antibody YM366Ed of DR50, and the detection method for ELISA of sDR5 was established. Results： The detection method for ELISA of sDR5 was established, the variation coefficient was less than 5%, the recovery rate was more than 85%, sDR5 serum concentration of 52 case of ehronic hepatitis B is higher than that of healthy persons. Conclusion： The detection method for ELISA ofsDR5 was established, whieh can be used to judge the patients condition, the effective rate of some medicines and the prognosis. The determination of sDR5 levels in patients with chronic hepatitis B could be used as index to indicate the activity of chronic hepatitis B.

关 键 词：ELISA sDR5 细胞凋亡 肝炎 病毒性 

分 类 号：R392.11[医药卫生—免疫学]