绵羊角蛋白关联蛋白KAP6-1基因5′端调控区的分子克隆及测序结果比较  

Molecular Cloning of 5′ Flanking Region of Ovine Keratin Associated Protein 6-1 Gene and Comparison of the Sequences

作　　者：郭旭东[1] 毛舒燕[1] 宝明涛[1] 尹俊[2] 旭日干[1] 

机构地区：[1]内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室,呼和浩特010021 [2]内蒙古农业大学生物工程学院,呼和浩特010018

出　　处：《内蒙古大学学报（自然科学版）》2007年第1期58-63,共6页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家863计划项目(2002AA242061);内蒙古自然科学基金(200607010405);内蒙古自治区高等学校科学研究项目(NJ06055)资助

摘　　要：根据绵羊毛囊角蛋白关联蛋白(KAP 6-1)基因已知DNA序列设计合成了两个特异性引物,以绵羊基因组DNA为模板,PCR扩增出1 042 bp的特异性片段,连接到pM D 19T载体中获得该片段克隆.经过快速提质粒法筛选、限制性内切酶分析表明该克隆包含所需的目的片段.DNA测序结果也证明该克隆片段与原基因5′端调控区序列相比具有很高的一致性.研究结果为今后制备转基因克隆动物,在毛囊细胞中特异性表达绒毛生长调控基因奠定了基础.Two specific oligonucleotide primers were designed and synthesized according to the sequence of ovine keratin associated protein 6-1 gene. Then a size of 1042 base pairs fragment was amplified by polymerase chain reaction. Subsequently the fragment was inserted into the EcoRV site of pMD19-T vector. The recombinant clonings were screened out by restriction analysis and were sequenced. The result showed the insertion has a high identity with original sequence and made it possible to obtain transgenic animal next step. Furthermore,the mechanism of hair regulatory genes which are expressed in hair follicle cells will be explored.

关 键 词：角蛋白关联蛋白6-1 毛囊细胞 调控区序列 

分 类 号：Q781[生物学—分子生物学]