睾丸特异性基因TSF22在小鼠中的表达研究  

Expression analysis of a testis-specific gene, TSF22, in mice

作　　者：唐爱发[1] 余振东[1] 桂耀庭[1] 郭新[1] 张立兵[1] 张键荣[1] 刘春霖[1] 蔡志明[1] 

机构地区：[1]北京大学深圳医院男性生殖医学实验室,深圳北京大学香港科技大学医学中心,深圳518036

出　　处：《中国男科学杂志》2007年第2期11-15,共5页Chinese Journal of Andrology

基　　金：国家自然科学基金资助项目(编号:30471728和30500543);广东省自然科学基金项目(编号:04007303)

摘　　要：目的筛选与精子发生相关的基因。方法将4、9、18、35、54日龄和6月龄小鼠睾丸组织cDNA探针与Affymetrix全基因组芯片进行杂交,筛选出差异表达的基因。利用网络信息资源对该基因进行生物学信息分析。RT-PCR分析该基因在小鼠睾丸不同发育阶段及小鼠不同组织中的表达。结果芯片结果分析筛选出一个差异表达杂交点(GenBank登录号:NM_199034),生物信息学分析发现该基因全长1597bp,含有570bp的完整ORF,编码190个氨基酸、分子量为22.106kDa的蛋白质,我们将其命名为TSF22。RT-PCR分析表明TSF22基因特异性表达于睾丸组织及18日龄小鼠睾丸中。结论TSF22基因的表达与小鼠精子发生的过程一致,可能在精子发生中起重要作用。Objective To identify genes involved in spermatogenesis. Methods Testes cDNA samples from Balb/C mice of different postnatal days were performed with mouse whole genome Affymetrix chip analysis to screen the testisspecific genes. The expression profile of the selected gene was also identified by RT-PCR. The characteristics of the selected gene were analyzed by various bioinformatic tools. Results By analysing the hybridization signals, a gene TSF22 with a differential expression in the developmental stages of testis was identified. The full length cDNA of 1597 bp contained an open reading frame of 570 bp which encoded a putative protein of 190 amino acids, this gene was named TSF22. RT-PCR analysis revealed that TSF22 mRNA was exclusively expressed in mice testis. Conclusion TSF22 functions may be as a testis-specific transcription factor that plays important roles during spermatogenesis.

关 键 词：精子发生 基因芯片 TSF22基因 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学] Q786[医药卫生—基础医学]