水稻和稻瘟病菌互作中的信号传导及防御反应基因诱导表达的研究  

Induced expression of signaling pathway and defense-related genes in interaction between rice and Magnaporthe grisea

作　　者：乐美旺[1] 陈实[2] 潘庆华[3] 曾任森[1] 刘迎湖[2] 骆世明[1] 

机构地区：[1]华南农业大学农学院 [2]华南农业大学理学院,广州510642 [3]华南农业大学资源环境学院,广州510642

出　　处：《植物病理学报》2007年第1期42-49,共8页Acta Phytopathologica Sinica

基　　金：国家重点基础研究发展计划"973"项目(2006CB100206);国家自然科学基金资助项目(30270230;30370246);广东省自然科学基金团队项目(E039254);广东省自然科学基金重点项目(04105977)

摘　　要：水稻和稻瘟病菌互作是研究植物与病原菌互作的模式体系。本文利用3个水稻抗稻瘟病近等基因品系(CO39、C101LAC和C101A51)和2个特异性菌株(M209和M210)构成不同亲和程度的互作关系,研究了稻瘟病菌对3个水稻信号传导途径关键酶合成基因、5个防御反应病程相关蛋白基因和1个防御反应转录因子调控基因诱导表达的作用。结果表明:稻瘟病菌诱导了各种互作关系中水稻OsLOX、OsAOS和OsPAL酶合成基因的表达,水稻启动了茉莉酸和水杨酸防御反应信号传导途径。在不亲和的互作反应中,稻瘟病菌能不同程度地诱导水稻OsPR1a、OsPR2、OsPR3-1、OsPR3-2和OsPR4基因的表达,从而有效激活了防御反应系统,使水稻植株表现为抗病;而在亲和的互作反应中,多数OsPR基因的表达水平低、时间短或没有表达,水稻植株表现为感病。OsMyb基因在各种互作关系中有不同的诱导表达。说明这些防御相关基因的诱导表达可能与水稻抗稻瘟病性相关。Interaction between rice （Oryza sativa L. ） and Magnaporthe grisea is a model system to study the interplays between plants and pathogens. The induced expressions of three key enzyme genes of plant signaling pathway, five pathogenesis-related protein genes, and one defense-related transcription factor control gene were studied in rice leaves by using three rice near-isogenic lines （CO39, C101LAC, C101A51） and two special blast isolates （M209, M210）. The results showed that OsLOX, OsAOS and OsPAL were induced by two special isolates in all interactions. Jasmonate-and salicylate-dependent defense signaling pathways were activated at the same time. While OsPR1a, OsPR2, OsPR3-1, OsPR3-2 and OsPR4 were expressed at higher levels in the leaves of incompatible rice plants and also for longer time than that in the leaves of compatible rice plants. OsMyb gene was induced differentially in different interactions between rice and M. grisea. Higher expression of these defense genes in incompatible rice plants might be responsible for resistance against M. grisea.

关 键 词：水稻 稻瘟病菌 信号途径 防御反应基因 诱导表达 

分 类 号：S435.111.2[农业科学—农业昆虫与害虫防治] S435.111.41[农业科学—植物保护]