侵染月季的李坏死环斑病毒的检测及CP基因序列分析  

Detection and sequencing of CP gene of Prunus necrotic ring spot virus in rose

作　　者：王继华[1] 王丽花[1] 杨秀梅[1] 苏艳[1] 张颢[2] 唐开学[1] 

机构地区：[1]云南省农业科学院农业部花卉产品质检中心,昆明650205 [2]云南省农业科学院花卉研究所,昆明650205

出　　处：《植物病理学报》2007年第1期99-101,共3页Acta Phytopathologica Sinica

基　　金：云南省科技攻关项目(2004NG08)

摘　　要：Rose leaves with virus symptoms(mosaic,ring spot and line pattern) were detected by DAS-ELISA and RT-PCR.The results showed Prunus necrotic ring spot virus(PNRSV) infection in the leaves.CP gene of PNRSV isolated from Yunnan province was cloned and sequenced.Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids(EMBL accession number: AY684271).Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group I isolates,and less than 95% between PNRSV-yunnan and group II and III isolates.According to the results,PNRSV-yunnan was identified as a member of group I.Rose leaves with virus symptoms （mosaic, ring spot and line pattern） were detected by DAS-ELISA and RT-PCR. The results showed Prunus necrotic ring spot virus （PNRSV） infection in the leaves. CP gene of PNRSV isolated from Yunnan province was cloned and sequenced. Analysis of sequence showed that CP gene of PNRSV-yunnan contained 683 nucleotides encoding 165 aminoacids （EMBL accession number： AY684271）. Nucleotide similarity of CP gene was more than 98% between PNRSV-yunnan and group Ⅰ isolates, and less than 95% between PNRSV-yunnan and group Ⅱ and Ⅲ isolates. According to the results, PNRSV-yunnan was identified as a member of group Ⅰ.

关 键 词：李坏死环斑病毒 基因序列分析 切花月季 监督检测 CP 侵染 VIRUS PNRSV 

分 类 号：S662.5[农业科学—果树学]