小麦抗叶锈病基因近等基因系TcLr35基因表达差异研究  

Differential genes expression of near-isogenic lines TcLr35 against wheat leaf rust

作　　者：陈云芳[1] 杨文香[1] 闫红飞[1] 刘力强[1] 褚栋[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《植物病理学报》2007年第1期109-112,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30170602)

摘　　要：The differential genes expression of adult plant resistance gene of TcLr35 was analysed by using cDNA-AFLP technique.The results showed that 33 of 843 amplified bands were differential ones in different growth stages of TcLr35.A polymorphic band amplified by primer pair E-TG/M-CTG was found at different leaf-growing stages from the second to the sixth leaf of TcLr35 and absent in Thatcher and the seedling stage of TcLr35 on TcLr35 without inoculated with Puccinia recondita.Reverse Northern blotting test suggested that band E-TG/M-CTG-192 was a piece of special gene fragment of TcLr35.The band was cloned and sequenced subsequently.The fragment of 192 bp produced by E-TG/M-CTG contained an open reading frame(ORF),but did not show homologous to the published wheat gene sequences.The differential genes expression of adult plant resistance gene of TcLr35 was analysed by using cDNA-AFLP technique. The results showed that 33 of 843 amplified bands were differential ones in different growth stages of TcLr35. A polymorphic band amplified by primer pair E-TG/M-CTG was found at different leaf-growing stages from the second to the sixth leaf of TcLr35 and absent in Thatcher and the seedling stage of TcLr35 on TcLr35 without inoculated with Puccinia recondita. Reverse Northern blotting test suggested that band E-TG/M-CTG-192 was a piece of special gene fragment of TcLr35. The band was cloned and sequenced subsequently. The fragment of 192 bp produced by E-TG/M-CTG contained an open reading frame （ORF）, but did not show homologous to the published wheat gene sequences.

关 键 词：基因表达差异 小麦产量 抗叶锈病基因 近等基因系 AFLP技术 RT-PCR 生长发育过程 抗性基因 

分 类 号：S512.1[农业科学—作物学]