鹅IGF-Ⅰ基因5’调控区序列的克隆与分析  

Molecular Cloning and Sequence Analyzing of Goose IGF-ⅠGene 5’-UTR

作　　者：王雷[1] 王宝维[1] 贾晓晖 杨志刚[1] 刘光磊[3] 张名爱[1] 张旭晖[1] 龙芳羽[1] 

机构地区：[1]莱阳农学院优质水禽研究所,青岛266109 [2]山东日照市畜牧局,日照276826 [3]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《中国畜牧兽医》2007年第3期71-73,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家"十五"科技攻关项目(2004BA514A09-3)

摘　　要：根据鸡、鸭等的IGF-Ⅰ基因5’调控区的保守区序列设计一对兼并引物,从五龙鹅的基因组DNA中扩增了IGF-Ⅰ基因5’调控区序列,将其克隆到pMD18-T载体上进行测序,结果得到长796bp的序列。五龙鹅IGF-Ⅰ基因5’调控区序列与鸭、鸡的同源性分别为98.1%、93.0%,充分显示了IGF-Ⅰ基因在进化上的高保守性;与鸭相比有4处碱基缺失和7处碱基插入;与鸡相比有4处碱基缺失和3处碱基插入。对其序列进行酶切图谱分析发现,共有5种常见的限制性内切酶的酶切位点。According to the IGF- I gene 5＇-UTR conserved sequences of chicken, duck etc, a pair of degenerate primer was designed to amplify the sequence of Wulong Goose IGF- I gene 5＇-UTR from the genomic DNA. And then the PCR product was subcloned to the pMD18-T vector and sequenced which was 796 bp in length. Compared with the duck and chicken, Wulong Goose IGF- I gene 5＇-UTR sequence shared 98.1 %and 93.0 %nucleotide homology respectively which indicated that IGF- I genes were highly conservative in phylogeny. Moreover, it has 4 bases deletion and 7 bases insertion compared to duck, and 4 bases deletion and 3 bases insertion compared to chicken. There are 5 kind of common restriction sites through nuclear acid DNA sequence by macrorestriction map analyzing.

关 键 词：IGF-I基因 5’调控区 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]