番茄内生细菌的分离及拮抗菌的筛选  

Isolation of Tomato Endophytic Bacteria and Selection of Antagonistic Strains

作　　者：王美琴[1] 陈俊美 薛丽[1] 贺运春[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]偏关县农业局,山西偏关036100

出　　处：《山西农业科学》2007年第2期55-58,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西农业大学创新基金项目(2004007);山西省科技攻关项目(033006)

摘　　要：从太谷、大同等地采集到3个不同品种的番茄健康植株,采用牛肉膏蛋白胨(NA)培养基从根、茎部共分离到65株内生细菌,通过对其中的31株进行平板对峙试验,筛选出对叶霉病菌和灰霉病菌有拮抗作用的菌株6株,占菌株总数的19.4%;对早疫病菌和枯萎病菌有拮抗作用的菌株7株,占菌株总数的22.6%。抑菌圈半径最大可达15 mm。按抑菌圈半径大小,将拮抗菌分为强、中、弱三类。Sixty-five endophytic bacterial strains were isolated from the root, rootstock and stem of healthy tomato plants collected from Datong and Taigu regions. 6 bacterial strains, about 19.4% of all tested strains, showed antagonistic activities against Botrytis cinerea and Fulvia fulva. 7 bacterial strains about 22.6% of all tested strains, have antagonistic activities against Fusarium Oxysporum and Alternaria solani. The longest radius of suppression was 15mm. Antagonistic bacteria were divided into strong, middle and weak for the radius of suppression.

关 键 词：番茄 内生细菌 拮抗作用 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]