产超广谱β-内酰胺酶大肠埃希菌和肺炎克雷伯菌Ⅰ类整合子及其与ESBLs基因关系的研究  

ClassⅠ Integron and Its Correlation with Genes Coding for ESBLs in ESBLs-producing Escherichia coli and Klebsiella pneumoniae

作　　者：付英梅[1] 张凤民[1] 张文莉[1] 张和光[2] 陈晓贝[1] 郭微媛[2] 马佳毓[1] 

机构地区：[1]哈尔滨医科大学微生物教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学第二附属医院,黑龙江哈尔滨150086

出　　处：《中华医院感染学杂志》2007年第3期241-245,共5页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金项目(30170816);黑龙江省卫生厅资助项目(2005007-007)

摘　　要：目的研究产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和肺炎克雷伯菌整合子分布及其在ESBLs基因水平转移中的作用。方法采用PCR方法检测230株产ESBLs和197株非产ESBLs大肠埃希菌和肺炎克雷伯菌Ⅰ类整合酶基因intⅠ,分析Ⅰ类整合子与细菌耐药性的关系,与blaSHV、blaCTX和blaTEM编码基因的关联及经质粒转移的情况。结果产ESBLs和非产ESBLs菌株中Ⅰ类整合酶扩增阳性例数分别是137例(59.6%)和48例(24.4%),两组整合子阳性率差异有统计学意义(P<0.01);整合子阳性株对喹喏酮类、氨基糖苷类以及二者同时耐药的比率较高,依次为82.5%、81.8%和71.5%;产ESBLs菌株中blaSHV、blaCTX和blaTEM基因与整合子经质粒共同转移的频率分别是59.5%、95.5%和69.5%。结论Ⅰ类整合子在产ESBLs大肠埃希菌和肺炎克雷伯菌中的分布明显高于非产ESBLs菌株,并参与产ESBLs菌株多重耐药性的形成;整合子与blaCTX经质粒共同转移的频率高于其他两类基因,提示CTX型酶具有更强的扩散优势。OBJECTIVE To survey the distribution of class Ⅰ integron in extended-spectrum beta-lactamases （ESBLs）-producing Escherichia coli and Klebsiella pneumoniae and its contribution in horizontal transfer of ESBLs genes. METHODS The presence of class Ⅰ integron among 230 ESBLs-producers and 197 non-ESBLs- producers of E. coli and K. pneumoniae were detected by PCR. The correlation and co-transfer between integron and genes coding for SHV, CTX and TEM were studied. RESULTS One hundred and thirty-seven （59. 6%） isolates were positive for int Ⅰ gene among ESBLs-producers, contrasted to 48 （24.4%） in non-ESBLs-producers （P〈0. 001）. Strains positive for int Ⅰ were related to multidrug resistance with higher resistant rates to aminoglycosides and/or fluoroquinolones. The rates of transconjugation of integron together with blaSHV, blaCTX and blaTEM were 59. 5%, 95. 5% and 69. 5%, respectively. CONCLUSIONS Distribution of class Ⅰ integron in ESBLs-producing strains is more predominant than that in non-ESBLs-producers. It contributes to multidrug resistance in ESBLs-producing strains, blaCTX Are more liable to transfer via plasmid along with class Ⅰ integron.

关 键 词：整合子 耐药基因 水平转移 

分 类 号：R378.99[医药卫生—病原生物学]