宁波地区阴沟肠杆菌质粒型AmpC酶基因研究及一种新型ACT-1基因的发现  

Plasmid-mediated AmpC β-Lactamases Genes in Enterobacter cloacae in Ningbo Area and Preliminary Discovery of a New ACT-1 Subtype:An Epidemic Surveillance

作　　者：陈洁[1] 王卫华[1] 毛雄英 许小敏[3] 黄志刚[4] 孙珺 汪丽[1] 吕婉飞[1] 

机构地区：[1]宁波大学医学院附属医院,浙江宁波315020 [2]宁波市医学科学研究所,浙江宁波315020 [3]宁波市第二医院,浙江宁波315010 [4]宁波市第一医院,浙江宁波315010 [5]宁波市李惠利医院,浙江宁波315010

出　　处：《中华医院感染学杂志》2007年第3期246-249,共4页Chinese Journal of Nosocomiology

基　　金：宁波市医学科技计划项目(2004040)

摘　　要：目的了解宁波地区阴沟肠杆菌临床分离株质粒AmpC酶基因型流行状况。方法用聚合酶链反应(PCR)检测阴沟肠杆菌中AmpC酶基因,并作核苷酸序列测定,与GenBank相应基因进行同源性分析。结果85株阴沟肠杆菌中,质粒AmpC酶基因阳性28株,阳性率32.9%,其中DHA和ACT-1基因阳性率分别为29.4%和3.5%;DHA基因与GenBank中的注册序列相同,而ACT-1基因核苷酸及氨基酸序列经BLAST分析无相同序列,已被GenBank收录。结论宁波地区临床分离的阴沟肠杆菌中,存在质粒AmpC酶基因流行,以DHA型为主,兼有ACT-1基因,该ACT-1基因为一种新发现的基因型。OBJECTIVE To investigate the prevalence and genotype of plasmid-mediated AmpC β-1actamases genes in Enterobacter cloacae strains isolated from Ningbo area. METHODS All isolated E. cloacae strains were subjected by polymerase chain reaction （PCR）, and some of the fragments produced by PCR were sequenced and analyzed. RESULTS For 85 strains, the total positive rate of plasmid-mediated AmpC genes was 32.9%, and the positive rates of DHA and ACT-1 genotypes were 29. 4% and 3. 5%, respectively. CONCLUSIONS The main genotype of plasmid mediated AmpC genes in Ningbo area is DHA, and the second is ACT-1 ; furthermore, the ACT-1 gene may be a new subtype that worth to be further studied.

关 键 词：阴沟肠杆菌 质粒 AMPC酶 基因 

分 类 号：R378.99[医药卫生—病原生物学]