神经生长因子对大鼠心肌损伤后交感神经去支配及巢蛋白表达的影响  

Effect of nerve growth factor on sympathetic denervation and Nestin expression after myocardial damage in rats

作　　者：李颖[1] 王铜[1] 周令望[1] 邹宁[1] 刘阳[1] 姜明春[1] 于维汉[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心克山病研究所,150081

出　　处：《中国地方病学杂志》2007年第2期152-155,共4页Chinese Jouranl of Endemiology

摘　　要：目的研究神经生长因子（NGF）预处理对异丙肾上腺素（Iso）致大鼠心肌急性损伤后交感神经去支配及巢蛋白（Nestin）表达的影响。方法雄性Wistar大鼠60只，随机分成3组：对照组、Iso损伤组和NGF预处理组．每组20只。观察心肌损伤的病理变化和血清肌酸激酶（CK）改变；免疫组化和RT～PCR方法检测心肌酪氨酸羟化酶（TH）和Nestin的表达。结果NGF预处理组较Iso损伤组心肌坏死面积明显减小（P〈0．05）。CK值在Iso损伤组最高，NGF预处理组次之，均高于对照组（P〈0．01）。TH染色阳性的交感神经纤维密度在Iso损伤组[（20．63±6．04）个／mm^2]明显下降，与对照组[（31．40±8．96）个／mm^2]比较，差异有统计学意义（P〈0．05），而NGF预处理组[（29．22±9．11）个／mm^2]TH阳性表达与对照组水平接近，并未发生显著减少。各组THmRNA表达水平与免疫组化结果平行。对照组NestinmRNA表达水平最低，Iso损伤组和NGF预处理组较高，以NGF预处理组为著。结论NGF预处理可拮抗Iso致大鼠心肌损伤的，心脏交感神经去支配，减轻Iso的心肌损伤作用，提示NGF可通过神经营养作用在心肌损伤修复过程中起重要作用。Objective To study the effect of nerve growth factor （NGF） on sympathetic denervation and Nestin expression after myocardial damage induced by Isoprenaline （Iso） in rats. Methods 60 Wistar male rats were classified into control, Iso and NGF groups to observe the pathological changes of myocardial damage and levels of serum creatine kinase （CK）. By immunohistochemistry （IHC） method and RT-PCR, the expression of tyrosine hydroxylase （TH） and Nestin were detected. Results The areas of the myocardial necrosis were significantly smaller in the NGF group than in the Iso group （P 〈 0.05）. The levels of CK in the NGF group were lower than that in the Iso group but higher than that in the control group （P 〈 0.01 ）. The density of sympathetic fibers that stained positive for TH was significantly lower in group Iso [total nerve number （20.63 ± 6.04）/mm^2] than that in control group [total nerve number （31.40 ± 8.96）/mm^2, P 〈 0.05]. The level of positive expression TH was not obviously decreased in group NGF [total nerve number （29.22 ± 9.11 ）/mm^2], which was close to that in the control group. The levels of TH mRNA expression in three groups were parallel with immunohistochemistry result. The level of Nestin mRNA expression was higher in the groups of NGF and Iso than that in control group, with that of control being the lowest that of group NGF the highest. Conclusions The NGF can inhibit sympathetic denervation after myocardial damage induced by Iso in rats and alleviate the damage of myocardium induced by Iso, indicating that NGF might play a significant role in the process of myocardial damage and reparation through the mechanisms of neurotrophy.

关 键 词：神经生长因子 巢蛋白 心肌疾病 异丙肾上腺素 

分 类 号：R686[医药卫生—骨科学]