RAPD分子标记技术用于实验兔品系鉴定的初探  

RAPD Analysis of the Genetic Specificity in Laboratory Rabbits

作　　者：赵立虎[1] 谢夏阳[1] 赵丹凤[1] 张春燕[1,2] 狄敏[1] 刘丽均[1] 李晟阳[3] 张守纯[3] 徐平[1] 

机构地区：[1]中国科学院上海实验动物中心 [2]南京210095 [3]沈阳农业大学畜牧兽医学院

出　　处：《上海交通大学学报（农业科学版）》2007年第1期66-70,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家科技自然资源条件平台工作项目(2004DEA0365)

摘　　要：本文选用新西兰白兔(New Zealand White Rabbit,NZWR)、青紫兰兔(Chinchilla Rabbit,CR)、日本白兔(Japanese White Rabbit,JWR)3个品系的血样提取基因组DNA,并应用随机引物扩增多态性DNA技术(Random Amplified Polymorphic DNA,RAPD)进行遗传分析和比较。结果表明,使用随机引物PCR扩增后,扩增产物经琼脂糖凝胶电泳检测共发现78个条带,其中多态性片段25条,占32.1%,从7套共140个随机引物中筛选出10个多态性较高的引物,包括BH9、AD1、BH11、AN12、AN14、O19、AD3、AD19、O4和O14。其中BH9、AD1扩增效果最好,扩增产物有较大的共性和较好的多态性,可作为实验兔品系鉴定的分子依据。日本白兔和新西兰兔片段共享度最高为0.8268,青紫兰兔和日本白兔最低为0.6190,统计结果显示RAPD标记多态性丰富,3品系的种内相似度大于种间相似度。该技术可用于实验兔的遗传检测。Rabbit genomic DNAs derived from Japanese White Rabbits （JWR）, Chinchilla Rabbits （CR） and New Zealand White Rabbits （NZWR） were analyzed with random amplified polymorphic DNA （RAPD） technique and the genetic variation among 3 rabbit strains were studied. The results showed that after 140 random primers in 7 sets were used to amplify the rabbit genomie DNA with PCR methods, 10 have been screened out on the preliminary experiments, including BH9,AD1,BH11,AN12,AN14,O19,AD3, AD19,O4 and O14. Two primers （AD1 and BH9） among the 10 are superior to others and could not only amplify the common DNA bands of 3 rabbit strain lines but also the different characteristic bands of each strain. 78 amplified fragments were detected by electrophoresis on agarose gel. Among them, 25 fragments were polymorphie, accounting for 32.1%. It was showed that the RAPD polymorphisms among rabbit lines were abundant. The genetic similarity between JW rabbits and NZW rabbits was highest （0.8286） among 3 rabbit strains and the lowest genetic similarity between CR and JW is 0.6190. The amplified band patterns also showed that the genetic similarity inner a strain is bigger than among different strains. The RAPD technique could be used as a method to monitor genetic relationship among different rabbit strains.

关 键 词：实验兔 RAPD 遗传检测 

分 类 号：Q95-33[生物学—动物学] S865.29[农业科学—野生动物驯养]