电针对去卵巢大鼠脑内雌激素受体mRNA的影响  

Effect of Electroacupuncture on Expression of Estrogen Receptors mRNA in the Brain of Ovariectomized Rats

作　　者：王伟[1] 姜明春[2] 尹岭[1] 田淑君[2] 

机构地区：[1]中国人民解放军总医院神经信息中心,北京市100853 [2]哈尔滨医科大学生理教研室,黑龙江哈尔滨市150086

出　　处：《中国康复理论与实践》2007年第3期233-235,共3页Chinese Journal of Rehabilitation Theory and Practice

摘　　要：目的探讨雌激素降低对脑内雌激素受体基因表达的影响以及电针刺激足三里穴对去卵巢大鼠脑内雌激素受体基因表达的调整作用。方法选用成年Wistar雌性大鼠,将动物分为正常对照组(INT)、去卵巢组(OVX)和去卵巢针刺组(OVX+EA)。用放射免疫分析方法测定血中雌二醇和睾酮的含量,采用RT-PCR方法获得大鼠雌激素受体α(ERα)和雌激素受体β(ERβ)mRNA的逆转录表达产物cDNA,用琼脂糖凝胶电泳方法检测,计算机图像分析系统进行统计分析。结果与对照组比较,去卵巢组大鼠血中雌二醇水平明显降低(P<0.01),同时伴有睾酮升高,脑内ERαmRNA的RT-PCR表达产物减少(P<0.01),ERβmR-NA的RT-PCR表达产物增加(P<0.01);与去卵巢组相比,去卵巢针刺组大鼠血中雌激素水平明显升高(P<0.01),睾酮水平下降(P<0.01),脑内ERαmRNA的RT-PCR产物升高(P<0.01),ERβmRNA的RT-PCR产物降低(P<0.01)。结论去卵巢大鼠血中雌激素水平降低,睾酮水平升高,脑内ERα和ERβmRNA的表达发生了明显的变化;电针足三里穴对体内雌激素和睾酮水平及脑内雌激素受体基因表达有明显的调整作用,这可能是电针调节神经内分泌功能的机制之一。Objective To determine the regulation of electroacupuncture （EA） at Zusanli acupoint for the expression of estrogen receptors mRNA in the brain of ovariectomized rats, Methods Female Wistar rats were randomly divided into 3 groups： intact group （INT）, ovariectomized group （OVX）, and ovariectomy and acupuncture group （OVX＋EA）. Radioimmunoassay （RIA） was used to measure the estradiol （E2） and testosterone （T） levels in plasma. The mRNA levels of estrogen receptor （ER）α and ERβ in the brain of rats were measured with RT-PCR. Results The levels of endogenous estrogen increased significantly in OVX＋EA rats compared with OVX rats （P〈0.01）, while the plasma T levels were lower in the OVX＋EA group than those of the OVX group （P〈0.01）. Eelectroacupuncture can up-regulate ERα expression in the brain of ovariectomized rats （P〈0.01）. However, it was shown to have a down-regulatory effect on the levels of ERβ expression （P〈0.01）. Conclusion There is an intimate relationship between the expressions of estrogen receptors mRNA and the endogenous hormone levels, such as E2 and T. Electroacupuncture can regulate the expression of estrogen receptors mRNA in the brain and the E2 and T levels in plasma of ovariectomized rats, which might, at least in part, explain the mechanisms of acupuncture adjusting the neuroendocrine system.

关 键 词：雌激素 性激素 雌激素受体 电针 去卵巢大鼠 

分 类 号：Q492.5[生物学—生理学] R339.3[医药卫生—人体生理学]