利用“02428h”的eui基因选育长穗颈不育系  

Breeding for PGMS Line by Using eui Gene from Recessive Tall Rice 02428h

作　　者：王才林[1] 赵凌[1] 朱镇[1] 张亚东[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心,江苏南京210014

出　　处：《作物学报》2007年第4期566-571,共6页Acta Agronomica Sinica

基　　金：农业结构调整重大技术研究专项(05-01-05B);农业部超级稻新品种选育与示范项目;江苏省高技术研究项目(BG2004304;BG2004327;BG2005301)

摘　　要：为研究隐性长穗颈水稻“02428h”的长穗颈基因eui在减轻不育系包颈度中的利用价值,以02428h为父本,培矮64S为母本和轮回亲本,经过连续3次回交将02428h的eui基因导入培矮64S,转育成了具有长穗颈基因的“P8hS”。与培矮64S相比,P8hS的株高增加35.6cm,主要是穗颈节和倒2节间长度增加所致。eui基因的导入不仅可使培矮64S的穗包颈得到彻底改善,而且对培矮64S及其杂种的主要经济性状具有改进作用。Elongated uppermost intemode was firstly found in 1981 by Rutger and Camahan. They named the recessive gene controlling this character as elongated uppermost intemode （eui） gene. It is regarded as the forth genetic factor besides CMS line, maintain line, and restoring line, improving panicle exsertion and reducing the applying amount of GA3 in three-line hybrid rice seed production. Since then, several eui mutants have been found. 02428h is one of such recessive tall rice mutants with eui from somaclonal variation of a wide compatibility japonica rice 02428. Pei＇ai 64S is a photo- and thermo-sensitive sterile line with stable sterility and good combining ability. It is widely used in rice breeding of two-line hybrid and some super high yielding rice hybrids such as Liangyoupeijiu （Pei＇ ai 64S/9311）. However, its panicle exsertion is not so good and 450-600 g ha^-1 GA3 must be applied in its seed production. In order to improve the panicle exsertion of photo- and thermo-sensitive sterile line ＂Pei＇ ai 64S＂, we conducted a breeding program transferring the eui gene into Pei＇ ai 64S through successive backcmssing by using eui gene of 02428h. In BC1F1 and BC2F1, the plant used for backcmssing was self pollinated and harvested for investigating the segregation of eui gene in next generation. A new photo- and thermo-sensitive sterile line ＂P8hS＂ with the character of elongated uppermost intemode was bred through three generations of successive backcmssing. Compared with Pei＇ ai 64S, P8hS had no difference in sterility and sterility period. The plant height of P8hS was 35.6 cm higher resulted from the elongation of the uppermost intemode and the second intemode from the top. The panicle extrusion of P8hS was completely improved with its particle neck length of 13.8 cm. The panicle length of P8hS was 2.2 cm longer and the number of spikelets per panicle was 35 more in P8hS than in Pei＇ ai 64S. The plant height was significantly higher, panicle length and panicle neck length were longer, the nu

关 键 词：水稻 隐性长穗颈 EUI基因 遗传 回交转育 

分 类 号：S511[农业科学—作物学]