食品污染产单核李斯特菌不同检测方法学评估  

Assessment of different methods in detection of Listeria monocytogenes in contaminated food products

作　　者：王冰[1] 扈庆华[1] 石晓路[1] 李庆阁[2] 郑琳琳[2] 林一曼[1] 张顺祥[1] 林世平[3] 邓辉萍[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518020 [2]厦门大学生命科学学院,福建厦门361005 [3]深圳市慢性病防治院,广东深圳518020

出　　处：《中国热带医学》2007年第4期506-507,570,共3页China Tropical Medicine

基　　金：国家自然科学基金资助项目(30300281);广东省卫生厅资助项目(A2003709);深圳市科技局资助项目(200404139)

摘　　要：目的评价以不同方法检测食品中污染产单核李斯特菌(LMO)效果,并对其检测方法进行优化。方法同时用国标法、改良国标法、改良分子信标-实时荧光PCR法、免疫磁性分离法对深圳市2004～2006年食品样品进行LMO分离鉴定并进行比较。结果228份样品中8份增菌液LMO实时荧光PCR阳性,其中6份LMO细菌培养阳性。国标法、改良国标法,改良分子信标-实时荧光PCR法、免疫磁性分离法都能检出LMO,检出率分别为2.63%、2.63%、3.50%、2.63%。深圳市食品中LMO的污染率为2.63%(国标法),速食类冻品污染率为12.8%(国标法)。结论荧光PCR法检出率最高,将检测时间由原来的至少4～5d缩短至1d,可用于食品污染LMO状况调查的初筛和食物中毒的快速诊断;国标法与改良国标法的检出率相等,而后者的操作更方便。免疫磁性分离法的检出率最低。Objective To investigate Listeria monocytogenes contamination in food of Shenzhen City. To find out a better method for detection of Listeria monocytogenes （LMO）. Methods Traditional method, the modified traditional method, the modified molecular beacons and real - time PCR and the immunomagnetic separation were used for isolation, identification of Listeria monocytogenes and the results were compared. Results LMO were detected by the traditional method, the modified traditional method, the modified molecular beacons and real-time PCR and the detection rates were 2.63% ,2.63% ,3.50% and 2.19%. LOM were detected from 8 samples by modified molecular beacons and real - time PCR. Among these 8 positive samples, LOM were detected. The detection rate of LMO in food in Shenzhen was 2.63 % （traditional method）. Conclusion Compared with other three methods, the modified molecular beacons and real- time PCR was the most sensitive. The detection time was reduced from at least five days to only one day. It can be employed for detection of LMO from large quantity of samples. The detection rate of the traditional method was equal to the modified traditional method, but the latter was easier. The detection rate of the immunomagnetic separation was the lowest, but the detection time of it was shorter than the traditional method and the modified traditional method. There the food has been contaminated with LMO in food in Shenzhen City and monitoring work in indicated.

关 键 词：产单核李斯特菌 改良分子信标 实时荧光PCR 免疫磁性分离 检测方法评估 

分 类 号：R517.7[医药卫生—内科学]