曲格列酮对THP-1巨噬细胞源性泡沫细胞ABCA 1表达及胆固醇流出的影响  

Effects of Troglitazone on ATP Binding Cassette Transporter A1 Expression and Cholesterol Efflux in THP-1 Macrophage-derived Foam Cells-derived Foam Cells

作　　者：甘淋[1] 彭家和[1] 鲁立[1] 申丽丽[1] 张迁[1] 江渝[1] 

机构地区：[1]第三军医大学生物化学及分子生物学教研室,重庆400038

出　　处：《现代生物医学进展》2007年第1期82-84,共3页Progress in Modern Biomedicine

摘　　要：目的:研究曲格列酮对THP-1巨噬细胞源性泡沫细胞ABCA1表达及胆固醇流出的影响。方法:采用液体闪烁计数法测定曲格列酮处理后THP-1巨噬细胞源性泡沫细胞胆固醇流出。用RT-PCR和Western blotting的方法检测曲格列酮处理后THP-1巨噬细胞源性泡沫细胞ABCA1 mRNA水平和蛋白质水平的变化。结果:经曲格列酮处理后,THP-1巨噬细胞源性泡沫细胞的胆固醇流出具有时间依赖性的增加,从0h的1．82%上升到24h的7．61%。在mRNA水平和蛋白质水平ABCA1也均随着曲格列酮作用时间增加而表达上调。结论:曲格列酮能增加THP-1巨噬细胞源性泡沫细胞ABCA1表达和胆固醇流出。Objective： To study the effect of Troglitazone on ATP binding cassette transporter A1 （ABCA1） expression and cholesterol efflux in THP-1 macrophage-derived foam cellss, Methods： After exposure of the cultured THP-1 macrophage- derived foam cells in Troglitazone for different times, cholesterol efflux was determined by liquid scintillator, ABCA1 mRNA and its protein level were determined by RT-PCR and Western blotting respectively. Results： The results showed that Troglitazone markedly increased ABCAl-mediated cholesterol efflux from THP-1 macrophage-derived foam cells, from Oh 1.82% to 24h 7.61%. This was accompanied by an increase membrane content of ABCA1, either in the level of protein or of mRNA. Conclusion： Troglitazone increases cholesterol efflux and the level of ABCA1 protein and mRNA in THP-1 macrophage-derived foam cells

关 键 词：胆固醇流出 ABCAl PPARΓ AS 

分 类 号：Q71[生物学—分子生物学]