逆转录－聚合酶链反应（RT－PCR）用于鉴别脊髓灰质炎病毒和其它肠道病毒  

RT一PCR FOR DIfFERENTIATION BETWEEN POLIOVIRUSES AND ENTEROVIRUSES

作　　者：孔健[1] 李宇静[1] 章美云[1] 徐爱强[1] 迮文远[1] 

机构地区：[1]卫生部北京生物制品研究所,山东省卫生防疫站

出　　处：《中国计划免疫》1996年第3期120-123,共4页Chinese Journal of Vaccines and Immunization

摘　　要：用一对选自脊髓灰质炎病毒及一对由Ｈｙｙｐｉａ所设计的细小ＲＮＡ病毒核酸５’端非编码区的引物，逆转录并扩增脊髓灰质炎病毒及其它肠道病毒的核酸，从而把脊髓灰质炎病毒和其它非脊髓灰质炎肠道病毒进行鉴别。１０５份被血清中和试验定型为脊髓灰质炎病毒的标本，逆转录一聚合酶链反应（ＲＴ─ＰＣＲ）的结果亦定为脊髓灰质炎病毒，灵敏度为１ｍ％；１３３份被血清中和试验定为非脊髓灰质炎肠道病毒的标本，均被判定为非脊髓灰质炎病毒，特异度为１００％。该方法可用于脊髓灰质炎病毒的检测及脊髓灰质炎病毒与其它非脊髓灰质炎肠道病毒的鉴别。or rapid distinguishing of polioviruses from other nonpolio-enteroviruses isolated from clini-cal specimens,a protocal was developed using a pair of primers selected from the conserved 5'noncoding region of the poliovirus genome and a pair of human picomavirus primers reported byHyypia.105 virus strains which were identified as polioviruses by tissue culture seroneutralizationtest were positive by polio-specific RT-PCR,the sensitivity was 100%.Of the 133 virus strainsidentified as nonpolio-enteroviruses by tissue culture seroneutmlizing test,no amplified productswere detected by polio-spndic RT-PCR,the specificity was 100%.It allowr for the detection ofpolioviruses and for distinguisiug then from nonpolio-enteroviruses.

关 键 词：脊髓在质炎 病毒 RT-PCR 肠道病毒 

分 类 号：R512.404[医药卫生—内科学] R512.504[医药卫生—临床医学]