在流式细胞计DNA测量中除去粘连细胞的方法  

Method for Removing Doublets aud Cell Aggregates in DNA Measurement with Flow Cytometry

作　　者：陶家平[1] 尹忆民[1] 

机构地区：[1]北京医科大学计算中心细胞分析室,l00083

出　　处：《中华物理医学杂志》1996年第3期169-171,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：为除去粘连细胞及细胞团对DNA测量的影响，应用小鼠胸腺细胞测量脉冲面积及宽度，在二维点图用圈除去粘控细胞。结果；DNA测量粘连细胞及细胞团严重影响G0/G1及M2+G2期测量，在固定细胞、实体病细胞等易产生粘连情况，细胞样品DNA测量中应用DDM除去粘连细胞的影响。By doublet discrimination module (DDM), area and width of fluorscence parameters in mousethymus cells were measured without influence of doublets and cell aggregates. The doublets and cellaggregates did seriously influence the measurement of G0/G1 and M2 + G2 phase in fixed cells on solid tissue cells easily to produce doublets of DNA DDM could remove influence of doublets in DNA measurement.

关 键 词：流式细胞计 辨别装置 粘连细胞 

分 类 号：Q503[生物学—生物化学]