DREB2A基因对苜蓿遗传转化的研究  

Genetic transformation of DREB2A gene into alfalfa

作　　者：盛慧[1] 朱延明[1] 李杰[1] 柏锡[1] 才华[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《草业科学》2007年第3期40-45,共6页Pratacultural Science

基　　金：黑龙江省科技厅重点攻关项目(GB05B104);中国博士后科学基金

摘　　要：以黑龙江紫花苜蓿Medicago sativa主栽品种:肇东苜蓿、敖汉苜蓿、公农1号和公农2号为受体材料,系统地探讨了除菌剂种类和浓度以及卡那霉素筛选浓度等,建立了高效的苜蓿再生体系和遗传转化体系;分别构建了由诱导型启动子rd29A和组成型启动子E12调控的DREB2A基因的2个植物表达载体pB2A29A和pB2AE12;采用农杆菌介导法进行遗传转化,获得大量转基因抗性植株并进行了分子生物学(PCR和Southern blot)检测。结果表明,DREB2A基因已整合到苜蓿基因组中。With ＂ZhaoDong＂, ＂AoHan＂, ＂No. 1 of GongNong＂, ＂No. 2 of GongNong＂ as researching material, the concentration and sorts of antibiotics and kanamycin sensitivity were systematically discussed, and high efficiency regeneration and genetic transformation system of alfalfa was established; constructed two plant expression vectors with DREB2A gene regulated by inducible expression promoter rd29A and constitutive expression promoter E12. Genetically transformed into alfalfa by way of Agrobacterium Tumefaciens and acquired a lot of transgenic plants and identified by molecule biology detection （PCR and Southern blot）. The result showed that, DREB2A gene had been joined into genome of alfalfa.

关 键 词：苜蓿 遗传转化 DREB2A基因 农杆菌介导 转基因植株 

分 类 号：S541.9[农业科学—作物学]