微渗析结合RP-HPLC研究盐酸平阳霉素在家兔血中的药代动力学  

Pharmacokinetics of pingyangmycin hydrochloride in rabbits determined by microdialysis coupled with RP-HPLC

作　　者：史健[1] 高子彬[2] 魏静[2] 丁平田[2] 陈大为[2] 

机构地区：[1]河北医科大学第四医院,河北石家庄050011 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《药学学报》2007年第3期297-300,共4页Acta Pharmaceutica Sinica

摘　　要：采用微渗析技术研究盐酸平阳霉素在体药代动力学，用Supelco RP—amide C16柱进行样品的HPLC分析，盐酸平阳霉素的血药浓度用3P87程序处理。结果表明，家兔静注盐酸平阳霉素后，渗析液中的药物浓度在1．04-66．56μg·mL^-1时，浓度与峰面积有良好的线性关系（r^2=0．9994）。微渗析探针的在体回收率为（42．8±3．4）％（n=6）。所测血药浓度经3P87程序拟合后，药一时曲线符合二室模型，T1/2α及T1/2β分别为14．9及60．3min。因此，该方法可用于盐酸平阳霉素药代动力学研究。Microdialysis coupled with RP-HPLC was used to study the blood pharmacokinetics of pingyangmycin hydrochloride in rabbits. Supelco RP-amide C16 column was adopted for the analysis of pingyangmycin hydrochloride. The data was analyzed with 3P87 program. The calibration curve was linear in the concentration range from 1.04 to 66.56 μg · mL^-1 （r^2 = 0.999 4）. The in vivo recovery of microdialysis probe was （42.8 ± 3.4 ） % （ n = 4 ）. The concentration-time curve of pingyangmycin hydrochloride was fitted to two-compartment model. T1/2 α and T1/2 β were 14.9 and 60.3 min, respectively. The method is proved to be accurate, simple and suitable for the pharmacokinetics study of pingyangmycin hydrochloride in rabbits.

关 键 词：盐酸平阳霉素 微渗析 反相高效液相色谱法 血药浓度 

分 类 号：R969.11[医药卫生—药理学]