肝癌患者手术后外周血AFP mRNA的检测及其与术后复发的关系  

Postoperative detection of AFP mRNA in the peripheral blood of hepatic cellular carcinoma patients and its correlation with recurrence

作　　者：智慧[1] 詹俊[2] 邓庆丽[4] 黄志明[3] 

机构地区：[1]中山大学附属第二医院超声科,广州510120 [2]中山大学附属第二医院消化内科,广州510120 [3]中山大学附属第二医院医学研究中心,广州510120 [4]中山大学生化教研室

出　　处：《中华肿瘤杂志》2007年第2期112-115,共4页Chinese Journal of Oncology

基　　金：国家“973”分课题资助项目（G1999054105）

摘　　要：目的探讨肝癌患者手术后外周血AFP mRNA的检测及其与术后复发的关系。方法原发性肝癌手术治疗患者29例，对照组为15例非原发性肝癌但须行肝部分切除手术者。分离术后第1，7和14天外周血单个核细胞，提取单个核细胞的总RNA，进行RT—PCR和Southem杂交，检测AFP mRNA，并对肝癌患者进行36个月的随访。结果全组有18例原发性肝癌患者复发。术后3次检测外周血AFP mRNA不全阴性者17例，其中14例复发，复发率为82．4％；3次检测均为阴性者12例，有4例复发，复发率为33．3％，差异有统计学意义（P＜0．05）。对照组手术后外周血AFP mRNA检测均为阴性。结论原发性肝癌患者手术后外周血AFP mRNA的检测可以预测肝癌的复发情况。Objective The postoperative presence of α-fetuprotien（AFP）mRNA in the peripheral blood of patient with hepatic cellular carcinoma （HCC） may suggest that carcinomatous cells still exist in the peripheral blood which may someday develop into metastasis. Therefore, the expression of AFP mRNA in postoperative peripheral blood is detected and its correlation with recurrence of HCC is investingated. Methods AFP mRNA in peripheral blood of HCC patients and non-hepatic cellular carcinoma patients treated by partial hepatectomy on the first, 7th, 14th postoperative day was examined by means of RT-PCR/ Southern blot. All HCC patients were followed up for 36 months. Results Of the 29 HCC patients, 18 （62. 1%） had recurrence, and 17 （58.6%） was found to have AFP mRNA expression in peripheral blood during postoperative period. Fourteen of these 17 patients （ 82.4% ） with positive AFP mRNA expression developed recurrence, but only 4 patients （33. 3% ） with negative AFP mRNA expression had recurrence. The difference between two groups was statistically significant （ P 〈 0. 05 ）. Conclusion Our preliminary results suggest that detection of AFP mRNA during postoperative period may be helpful in predicting recurrence for hepatic cellular carcinoma.

关 键 词：肝肿瘤 AFP MRNA 肿瘤复发 RT—PCR Southem杂交 

分 类 号：R686[医药卫生—骨科学]