HPLC法测定小儿止痒胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的含量  

Quantitative determination of cimicifugoside and 5-omethylvisammios-De in Xiaoerzhiyang Capsules by HPLC

作　　者：刘君焱[1] 凌宇静[2] 孙秀华[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国中医药报社

出　　处：《北京中医药大学学报》2007年第1期58-60,共3页Journal of Beijing University of Traditional Chinese Medicine

摘　　要：目的建立HPLC法测定小儿止痒胶囊中防风所含升麻素苷和5-O－甲基维斯阿米醇苷含量的方法。方法采用Diamonsil（TM）钻石C18（250mm×4．6mm，5μm），甲醇-水（40：60）为流动相；流速为1．0mL／min，检测波长为254nm。结果升麻素苷在0．3888—0.9072μg范围内线性良好（r=0．9999），5-O-甲基维斯阿米醇苷在0．1272—0．7632μg范围内线性良好（r=0．9996）。升麻素苷平均回收率为102．3％，RSD为2．59％（n=5）；5-O-甲基维斯阿米醇苷平均回收率为98．1％，RSD为1．53％（n=5）。结论本方法简便，结果准确。Objective To establish a HPLC method for quantitatively determining eimicifugoside and 5-o-methylvisammioside in Raidix Saposhnikoviae of Xiaoerzhiyang Capsules. Method The mobile phase was methanol-water（40： 60） and the detection wavelength was 254 nm. Results There was a good linear relationship for eimieifugoside from 0. 388 8 μg to 0. 907 2 μg（ r = 0. 999 9 ） and for 5- o-methylvisammioside from 0. 127 2 μg to 0. 763 2 μg（ r = 0. 999 6 ）. The average recovery of the procedure was 101.4% （ RSD = 1.62% ）. Conclusion The method is accurate and easy to be performed.

关 键 词：小儿止痒胶囊 升麻素苷 5-O-甲基维斯阿米醇苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]