改进的大鼠单发佐剂性关节炎模型的建立和实验观察  

A LIMITED ARTHRITIC MODEL FOR CHRONIC PAIN STUDIES IN RATS

作　　者：刘红香[1] 许伟[1] 罗非 王金树 韩济生 

机构地区：[1]河南医科大学生理教研室,北京医科大学神经科学研究中心,北京医科大学第三临床医院放射科

出　　处：《中国疼痛医学杂志》1996年第4期223-228,共6页Chinese Journal of Pain Medicine

摘　　要：本实验用油包水型完全弗氏佐剂（ＣＦＡ）进行大鼠单侧踝关节腔内注射，制备出一种单发性关节炎模型，对此模型动物进行的实验观察表明：注药侧踝关节在注药后几小时即开始出现急性红肿，３周后肿胀稳定于一定水平，保持６周以上；注药侧踝关节的屈、伸关节疼痛试验评分明显升高，踝关节压痛阈值显著降低，即出现了明显的患侧关节痛敏，并可稳定持续几周。注射佐剂后第４周和第６周进行的Ｘ线检测显示：注射ＣＦＡ的踝关节关节囊肿胀，关节腔狭窄，关节面粗糙。未见明显的关节周围骨质密度的改变，未发现动物身体其它关节出现变化。体重能稳定增长。以上结果提示：关节腔内注射油包水型完全弗氏佐剂引起的单发性关节炎大鼠全身状态良好，踝关节的炎性病变局限、固定、较轻，而关节局部痛敏明显，持续时间长，而且稳定。是一种比较好的慢性痛动物模型。Injection of water-in-oil type complete Freund's edjuvant(CFA,0.03ml)con taining 300μg dead mycobacterium tuberculosis(Human strain)into the tibio-tarsal joint pro duced a reliable, reproducible monoarthritic model.As revealed by behavioral observationsand X-ray examinations,the arthritis produced was anatomically lodlized,mild but prol onged and stable for several weeks.There was a marked increase in the sensitivity of theankle joint to lateral pressure and increased scores of arthritic fiexion and extension pain testin the affected limb,indicating the existence of hyperalgesia of the ankle joint. Animals de picted steady increase of body weight and remained active as opposd to polyarthritic ratswhere systemic disturbance of they functions are obvious.The results suggest that thismonoarthritis can be served as a chronic pain model for research purpose.

关 键 词：单发性 关节炎 动物模型 疼痛 CFA 

分 类 号：R684.3[医药卫生—骨科学] R441.1[医药卫生—外科学]