人脑颞叶组织中类固醇5a－还原酶同功酶活性分布研究  

Study on Distribution of Steroid 5a-Reductase Isozymes in Human Temporal Lobe

作　　者：郭华北[1] 林厚怡[1] 余裕民[1] 徐愤 蒋秉坤[1] 

机构地区：[1]蚌埠医学院临床生化教研室,蚌埠医学院核医学教研室,南京医科大学生化室

出　　处：《标记免疫分析与临床》1996年第3期134-137,共4页Labeled Immunoassays and Clinical Medicine

基　　金：联合国世界卫生组织(WHO)奖学金

摘　　要：本文采用酶放射分析法对新鲜人脑颞叶组织中５ａ－还原酶同功酶的活性分布进行研究。结果显示：（１）在颞叶脑组织中，５ａ－还原酶１和５ａ－还原酶２主要分布在灰质，其酶活性（５ａ－还原酶１，３３．６±４．５ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１２；５ａ－还原酶２，１３．８±２．９ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１１）明显高于分布在白质中的酶活性（５ａ－还原酶１，１４．７±２．０ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１２，Ｐ＜０．００１；５ａ－还原酶２，５．２±０．９ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝１１，Ｐ＜０，０１）；（２）在灰质中，５ａ－还原酶活性主要来自于５ａ－还原酶１，其酶活性（３４．９±２．５ｐｍｏｌ·ｈ_（－１）／ｍｇ蛋白，ｎ＝３２）明显高于５ａ－还原酶２（１５．０±２．３ｐｍｏｌ·ｒ_（－１）／ｍｇ蛋白，ｎ＝１８，（Ｐ＜０．００１）；（３）５ａ－还原酶１和５ａ－还原酶２的活性在男女性之间差异不显著，且与年龄无相关关系。The distribution of steroid 5a-reductase isoenzymes in human temporal lobe was studied by a radiometric method of enzyme assay. The results indicated that (1) 5a-reductase 1 and 5a-reductase 2 were mainly concentrated in the cerebral cortex with an activity of 33. 6±4. 5pmol· h(-1)/mg protein ( n = 12) and 13. 8±2.9pmol ·h(-1)/mg protein ( n = 11 ),respctively,which was significantly higher than that of 5a-reductase 1 (14. 7± 2. 0pmol · h(-1)/mg protein, n = 12, P <0. 001 and 5a-reductase 2(5. 2±0.9pmol · h(-1)/mg protein, n = 11, P <0. 01) in the white matter; (2)The predominant isoenzyme in the cerebral cortex was 5a-reductase 1,whose activity was 34. 9±2.5pmol. h(-1)/mg protein, ( n = 32),significantly higher than that of 5a-reductase 2 (15. 0± 2.3pmol. h(-1)/mg protein, n = 18, P <0. 001 ); and (3) There was no sex-related differences in both isoenzymes and no correlation between age and isozyme activity in human temporal lobe.

关 键 词：脑组织 同功酶 颞叶 5Α-还原酶 

分 类 号：Q554[生物学—生物化学] Q593.2