山西冬小麦品种Waxy蛋白分析及分子标记研究  

Analysis of Waxy Proteins in Shanxi Winter Wheat Cultivars Using SDS-PAGE and Molecular Markers

作　　者：王海萍[1] 唐朝晖[1] 刘少翔[2] 张兰萍[2] 逯成芳[2] 

机构地区：[1]山西大学生物工程学院,山西太原030006 [2]山西省农业科学院作物遗传研究所,山西太原030031

出　　处：《华北农学报》2007年第1期98-102,共5页Acta Agriculturae Boreali-Sinica

基　　金：山西省科技攻关项目(041003-2);山西省农科院高新技术项目(YGX0401)

摘　　要：利用聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析了山西100份小麦品种(系)Waxy蛋白的缺失类型,筛选出缺失Wx-B1蛋白亚基的材料8份。利用2个STS标记和1个SSR标记对不同Waxy蛋白缺失类型进行鉴定,验证其在分子标记辅助育种中的有效性,结果表明:Wx-7A位点的STS引物在野生型中扩增出1条450 bp的特异带,而在缺失Wx-A1蛋白亚基的突变材料中扩增出1条327 bp的特异带;Wx-4A位点的STS引物在野生型中扩增出1条440 bp的特异带,缺失Wx-B1蛋白亚基的突变材料中没有该扩增片段;Wx-7A,Wx-7D位点的SSR引物在野生型中分别扩增出1条265 bp和1条204 bp的特异带,在缺失Wx-A1和Wx-D1蛋白亚基的突变材料中没有扩增出该特异带。这3个标记可以用于分子标记辅助育种。A total of 100 wheat cuhivars and advanced lines from Shanxi were screened for the Waxy protein by SDS- PAGE. The results indicated that 8 of these cultivars were the null Wx-B1 type. Two STS and one SSR markers were used to analyze the wheat cuhivars with different types of Waxy proteins. Validation of the markers was carried out for the detection of Wx-7A, Wx-4A and Wx-7D genes. The resuhs indicated that a 450 bp-fragment was amplified with Wx-7A specific STS marker from the wild genotypes, while the fragment was 327 bp for the mutant with null Wx-A1 ; a 440 bpfragment was detected with Wx-4A specific STS marker from the wild genotypes, whereas, the fragment was absent for the mutant with null Wx-B1 ; a 265 bp-fragment and a 204 bp-fragment were detected with Wx-TA, Wx-TD specific SSR marker from the wild genotypes, while the fragments were absent for the mutant with null Wx-A1 and Wx-D1. These markers are useful tools to identify wheat cuhivars with mutant and normal alleles of the Waxy genes in marker-assisted selection of wheat breeding programme.

关 键 词：普通小麦 WAXY蛋白 聚丙烯酰胺凝胶电泳 STS标记 SSR标记 

分 类 号：S512.01[农业科学—作物学]