切除颌下腺对大鼠睾酮和精子顶体酶活力的影响  

Effect of extirpation of submandibular gland on weight of testis and epididymis, plasma testosterone and acrosin activity in rat

作　　者：冷双英[1] 范莉英[1] 陈琼 王更新[1] 赵岩[1] 赵春芳[1] 

机构地区：[1]河北医科大学

出　　处：《生殖医学杂志》1996年第3期158-161,共4页Journal of Reproductive Medicine

基　　金：河北省自然科学基金

摘　　要：本实验对大鼠切除颌下腺３０和４８天时，睾丸和附睾重量、血清睾酮水平及精子顶体酶活力进行了观察。结果显示：（１）切除颌下腺的实验组，睾丸和附睾重量明显低于对照组（Ｐ＜０．０５，Ｐ＜０．０１），睾丸脏器系数也明显降低（Ｐ＜０．０１），但附睾脏器系数无明显降低（Ｐ＞０．０５）；（２）血清睾酮水平３０天时实验组略低于对照组，４８天时为２．４１ｎｍｏｌ／Ｌ，明显低于对照组的４．４９ｎｍｏｌ／Ｌ（Ｐ＜０．０１）；（３）实验组的精子顶体酶活力分别为７．１９和６．００ｍＵ／ｍｌ明显低于对照组（Ｐ＜０．０１）分别降低了５５．１％和６１．１％。结果表明表皮生长因子（ＥＧＦ）参与了调节睾酮的分泌，并能影响精子顶体酶活力以及睾丸和附睾的重量。Objective: To investigate the changes in weight of testis and epididymis,plasma testosterone and acrosin activety after extirpation of submandibular gland in rats. Design: Animal experimental study. Results: (1)The weight of testis and epididymis organic coefficient and after operation decreased notably ( P<0.05,P <0.01).The organic coefficient of testis also decreased, but that of epididymis did not changed apparently ( P >0.05).(2)On day 30 after the operation, there was slight decrease in plasma testosterone. On day 48, the level of testosterone was 2.41 nmol/L, reduced significantly than that of the control group (4.49 nmol/L) ( P <0.01). (3)Acrosin activities of sperms were 7.19 mU/ml and 6.00 mU/ml respectively on day 30 and 48 after operation, which were decreased significantly by 55.1% and 61.1%,as compared with control group ( P <0.01). Conclusion: Submandibular gland, which contains large amount of growth factors, such as EGF, may regulate the secretion of testosterone and affect the acrosin activity and weight of testis and epididymis.

关 键 词：表皮生长因子 颌下腺 睾酮 精子顶体酶 男性不育 

分 类 号：R698.2[医药卫生—泌尿科学]