经冠状动脉移植自体骨髓单个核细胞和间充质干细胞对急性心肌梗死模型心功能的改善  

Intracoronary autologous bone marrow mononuclear cells and mesenchymal stem cells for improving the cardiac function of acute myocadial infarction models

作　　者：李树仁[1] 齐晓勇[1] 刘会良[1] 孟寸良[1] 王天红[1] 党懿[1] 张建清[1] 吴迪[1] 董洁[1] 李英肖[1] 郜利会[1] 靳福昌[1] 

机构地区：[1]河北省人民医院心脏中心,河北省石家庄市050051

出　　处：《中国组织工程研究与临床康复》2007年第11期2081-2086,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:为保护濒死心肌提供机会窗口,对比观察经冠脉移植自体骨髓单个核细胞或间充质干细胞后,实验性急性心肌梗死动物心功能变化及心肌组织核转录因子кB、心肌细胞凋亡情况。方法:实验于2005-03/2006-11在河北省人民医院实验中心完成。选用24只雄性冀中白猪,随机数字表法分为4组:正常对照组、模型组、单个核细胞组、间充质干细胞组,6只/组。①24只猪均以盐酸氯胺酮200mg臀部肌肉注射麻醉后,分别于各自右侧股骨抽取骨髓20mL,采用Fercoll法分离获得骨髓单个核细胞,加入胶体金溶液,培养12～16h待用。分离过程中取出含有骨髓单个核细胞成分的细胞层,常规培养传代,每3d换液1次,贴壁生长细胞即为骨髓间充质干细胞,加入胶体金溶液,培养24h待用。②除正常对照组外,其余各组均经导管球囊封闭第一对角支以远的前降支,复制猪急性心肌梗死模型。单个核细胞组、间充质干细胞组均于造模后立即开通前降支,分别经球囊注入预先分离的骨髓单个核细胞6×108个、间充质干细胞6×108个。模型组造模后于梗死1h开通前降支,经球囊注入磷酸盐缓冲液10mL。③各组分别于术前及术后4周经心脏超声检测心功能,取材行病理学检查、心肌组织核转录因子кB的免疫组织化学检测及心肌细胞凋亡检测。结果:24只雄性白猪均进入结果分析。①心功能变化:术前各组左心室收缩末内径、左心室舒张末内径、左心室射血分数、短轴缩短率基本相似。移植术后4周,正常对照组、单个核细胞组、间充质干细胞组左心室舒张末内径均明显低于模型组(F=4.68,P=0.01),左心室射血分数及短轴缩短率均明显高于模型组(F=5.14,P=0.01;F=3.32,P=0.04),各组左心室收缩末内径差异无显著性意义(F=1.64,P=0.21)。②心肌组织病理学改变:电镜下单个核细胞组、间充质干细胞组在梗死边缘区可见有胶体金颗粒的�AIM： To protect the ischemic myocardium to be infarct, we study the effects of intracoronary autologous bone marrow mononuclear calls and mesenchymal stem cells on the cardiac function and the changes of myocardium nuclear factor （NF）-κB and myocardium apoptosis of acute myocardial infarction. METHODS： The experiment was undertaken in Experimental Center, Hebei Provincial People＇s Hospital from March 2005 to November 2006. Twenty-four male white swines were selected and randomly divided into 4 groups, namely normal control group, model group, bone marrow mononuclear calls group and mesenchymal stem cells group, 6 in each group. ①Twenty-four swines was anaesthetized by intramuscularly injection 200 mg Ketamine, then 20 mL marrow was extracted from right thighbone. According Fercoll method autologous bone marrow mononuclear cells were gotten and mixed with colloid gold solution, cultured for 12-16 hours. Intercellular layer containing bone marrow mononuclear cells was obtained in separation process. Routine culture and passage was performed, change the liquor once every 3 days. Adhered calls were mesenchymal stem cells and mixed with colloid gold solution, cultured for 24 hours. ②Except normal control group, transcatheter blocking anterior descending branch made acute myocardial infarction models. Anterior descending branch was opened after establishing models, and 6×10^8 bone marrow mononuclear cells and 6×10^8 mesenchymal stem cells were infused into the criminal artery through Over-The-Wire balloon in the bone marrow mononuclear calls group and mesenchymal stem cells group. One hour after acute myocardial infarction anterior descending branch was opened in the model group. 10 mL phosphate buffer saline was inserted into sacculus sphaericus. ③Cardiac function ultrasound, pathology, immunohistochemistry of myocardial NF-κB and myocardial apeptosis were performed before operation and 4 weeks after operation in each group. RESULTS： Twenty-four male white swines entered the result analysis. ①c

关 键 词：自体骨髓单个核细胞 急性心肌梗死模型 骨髓间充质干细胞 心功能变化 冠状动脉移植 实验性急性心肌梗死 左心室舒张末内径 核转录因子κB 

分 类 号：R746.4[医药卫生—神经病学与精神病学]