血小板膜糖蛋白表达变化在出血性血小板病患者与健康人之间的差异(英文)  

Comparison of the changes in platelet membrane glycoproteins between patients with hemorrhagic thrombopathy and healthy people

作　　者：沈霖[1] 卢芙蓉[1] 沈迪[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,湖北省武汉市430022

出　　处：《中国组织工程研究与临床康复》2007年第11期2182-2184,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30572441)~~

摘　　要：背景:已证实出血性血小板病患者血小板聚集功能存在缺陷,血小板膜糖蛋白在血小板聚集过程中具有重要作用。目的:以健康人作为正常对照,观察出血性血小板病患者血小板膜糖蛋白表达的变化。设计:病例-对照分析。单位:华中科技大学同济医学院附属协和医院中西医结合科。对象:①选取2001-01/2003-03华中科技大学同济医学院附属协和医院中西医结合科和血液专科收治的79例出血性血小板病患者,男31例,女48例,平均年龄(35.76±14.14)岁,病程1～14年,符合1996年出血性血小板病的诊断标准,患者及其家属对本实验知情同意。共计167个出血部位,其中肢体皮肤瘀斑或瘀点64例,鼻出血33例,月经过多29例,齿龈出血28例,眼底出血8例,球结膜出血5例。②纳入标准:均有多部位出血临床表现;血小板计数、出血时间、凝血象检测无明显异常改变;根据出血部位,经内科、耳鼻喉科、妇科、口腔科和眼科等科室诊视,未发现特异诊断性病灶;血压、心率正常;经瑞斯托霉素检测,排除血小板无力症。③另选取健康献血员34例作为正常对照组,男15例,女19例,平均年龄(30.12±7.14)岁。方法:①血小板聚集率测定:以二磷酸腺苷(终浓度1.90μmol/L)、花生四烯酸(终浓度0.37μmol/L),血小板活化因子(终浓度150nmol/L)作为诱聚剂,采用Chronolog430型血小板聚集仪检测正常对照与出血性血小板病患者的血小板最大聚集率。血小板最大聚集率21%～40%为反应不良,低于20%为反应缺如。②血小板膜糖蛋白检测:患者取肘静脉血3.6mL,20g/L的乙二胺四乙酸二钠1:9抗凝,正常对照组标本以同法收集。分离血浆,收集血浆层,离心去上清,加多聚甲醛室温固定,调整血小板浓度为3×107L-1。取调整好的血小板溶液100μL,分别加入异硫氰酸荧光黄标记的CD42b(抗血小板膜糖蛋白Ⅰb)、CD41(抗血小板膜糖蛋白Ⅱb)、CD61(抗血小板膜糖蛋白BACKGROUND ： thrombopathy, It has been confirmed that platelet aggregation defect presents in patients with hemorrhagic and platelet membrane glycoproteins play a significant role in the process of platelet aggregation OBJ ECTIVE： To observe the expression changes of platelet membrane glycoproteins between patients with hemorrhagic thrombopathy and healthy people. DESIGN ： Case-control analysis SETTING： Department of Integrated Traditional Chinese and Westem Medicine, Union Hospital, Tongji Medical College Huazhong University of Science and Technology. PARTICIPANTS：① Seventy-nine patients with hemorrhagic thrombopathy who received treatment from January 2001 to March 2003 in the Department of Integrated Traditional Chinese and Westem Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology were enrolled in this study. The patients, including 31 male and 48 femlae, averaged （35.76＋14.14）years old. They all agreed to participate in this study and met with the diagnosis of hemorrhagic thrombopathy revised in 1996. Informed consents were obtained from all the patients and their relatives. The course of multiple bleeding symptoms was 1 to 14 years. And the bleeding sites were totally 167： extremity petechia was found in 64 cases,, rhinorrhagia in 33 cases, hypermenorrhea （more than 150 mL in each cycle） in 29 cases, gingival bleeding in 28 cases, ocular fundus bleeding in 8 cases and conjunctival hemorrhage in 5 cases.②Inclusive criteria： All the cases presented clinical manifestation of multiple focal bleeding; The laboratory tests including platelet counts, bleeding time and coagulation profiles were examined with no marked abnormal changes; According to hemorrhage sites, the patients were examined by specialists of intemal medicine, otorhinolaryngology, gynecology, stomatology and ophthalmology, and no specific diagnostic focuses of infection were found; They had normal blood pressure and heart rate, and those with thrombocytasthenia had been ruled

关 键 词：血小板膜糖蛋白类 血小板聚集 血小板疾病 P选择素 

分 类 号：R543[医药卫生—心血管疾病]